Gene description for Htra1
Gene name HtrA serine peptidase 1
Gene symbol Htra1
Other names/aliases AI429470
HTRA
L56
Prss11
RSPP11
Species Mus musculus
 Database cross references - Htra1
ExoCarta ExoCarta_56213
Vesiclepedia VP_56213
Entrez Gene 56213
UniProt Q9R118  
 Htra1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Mov neuroglial cells 15210972    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Htra1
Molecular Function
    serine-type endopeptidase activity GO:0004252 IBA
    serine-type endopeptidase activity GO:0004252 IEA
    protein binding GO:0005515 IPI
    serine-type peptidase activity GO:0008236 IDA
    growth factor binding GO:0019838 IEA
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    molecular function activator activity GO:0140677 ISO
Biological Process
    placenta development GO:0001890 IMP
    proteolysis GO:0006508 IBA
    proteolysis GO:0006508 IDA
    programmed cell death GO:0012501 IBA
    negative regulation of transforming growth factor beta receptor signaling pathway GO:0030512 IDA
    negative regulation of BMP signaling pathway GO:0030514 IDA
    positive regulation of apoptotic process GO:0043065 IBA
    positive regulation of epithelial cell proliferation GO:0050679 ISO
    negative regulation of defense response to virus GO:0050687 ISO
    chorionic trophoblast cell differentiation GO:0060718 IMP
Subcellular Localization
    extracellular region GO:0005576 IEA
    cytosol GO:0005829 IEA
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    collagen-containing extracellular matrix GO:0062023 HDA
    collagen-containing extracellular matrix GO:0062023 IBA
    collagen-containing extracellular matrix GO:0062023 ISO
 Experiment description of studies that identified Htra1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 39
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|HSC70|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors "Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G"
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Htra1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kctd13  
Affinity Capture-MS Mus musculus
2 Gdf5  
Affinity Capture-Western Mus musculus
3 Tgfb1 21803
Affinity Capture-Western Mus musculus
4 Tia1  
Affinity Capture-MS Mus musculus
5 Inhba  
Affinity Capture-Western Mus musculus
6 Bmp4  
Affinity Capture-Western Mus musculus
7 Tgfb2  
Affinity Capture-Western Mus musculus
View the network image/svg+xml
 Pathways in which Htra1 is involved
PathwayEvidenceSource
Degradation of the extracellular matrix IEA Reactome
Extracellular matrix organization IEA Reactome





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