Gene description for PROC
Gene name protein C (inactivator of coagulation factors Va and VIIIa)
Gene symbol PROC
Other names/aliases APC
PC
PROC1
THPH3
THPH4
Species Homo sapiens
 Database cross references - PROC
ExoCarta ExoCarta_5624
Vesiclepedia VP_5624
Entrez Gene 5624
HGNC 9451
MIM 612283
UniProt P04070  
 PROC identified in sEVs derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
 Gene ontology annotations for PROC
Molecular Function
    serine-type endopeptidase activity GO:0004252 IBA
    serine-type endopeptidase activity GO:0004252 IMP
    serine-type endopeptidase activity GO:0004252 TAS
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
Biological Process
    proteolysis GO:0006508 NAS
    blood coagulation GO:0007596 IBA
    negative regulation of blood coagulation GO:0030195 IBA
    negative regulation of blood coagulation GO:0030195 TAS
    negative regulation of apoptotic process GO:0043066 IMP
    negative regulation of inflammatory response GO:0050728 IMP
    negative regulation of coagulation GO:0050819 IMP
    positive regulation of establishment of endothelial barrier GO:1903142 IMP
Subcellular Localization
    extracellular region GO:0005576 NAS
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 TAS
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum lumen GO:0005788 TAS
    Golgi apparatus GO:0005794 IDA
    Golgi lumen GO:0005796 TAS
 Experiment description of studies that identified PROC in sEVs
1
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PROC
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 WNT5A 7474
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 EPHA6 285220
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 PROCR 10544
Reconstituted Complex Homo sapiens
4 SERPINA5 5104
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
5 PROS1 5627
Affinity Capture-MS Homo sapiens
6 UQCRH 7388
Affinity Capture-MS Homo sapiens
7 NPB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 CSNK2B 1460
Two-hybrid Homo sapiens
9 TK1 7083
Two-hybrid Homo sapiens
10 CYSRT1 375791
Two-hybrid Homo sapiens
11 PF4 5196
Reconstituted Complex Homo sapiens
12 PDF  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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