Gene description for Ube2d2a
Gene name ubiquitin-conjugating enzyme E2D 2A
Gene symbol Ube2d2a
Other names/aliases 1500034D03Rik
Ubc2e
Ube2d2
ubc4
Species Mus musculus
 Database cross references - Ube2d2a
ExoCarta ExoCarta_56550
Vesiclepedia VP_56550
Entrez Gene 56550
UniProt P62838  
 Ube2d2a identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Ube2d2a
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 ISO
    ubiquitin-protein transferase activity GO:0004842 ISS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ubiquitin protein ligase activity GO:0061630 IEA
    ubiquitin conjugating enzyme activity GO:0061631 IBA
    ubiquitin conjugating enzyme activity GO:0061631 ISO
Biological Process
    protein polyubiquitination GO:0000209 ISO
    ubiquitin-dependent protein catabolic process GO:0006511 IBA
    protein ubiquitination GO:0016567 ISO
    protein autoubiquitination GO:0051865 IEA
    protein autoubiquitination GO:0051865 ISO
    protein K48-linked ubiquitination GO:0070936 IBA
    protein K48-linked ubiquitination GO:0070936 IEA
    protein K48-linked ubiquitination GO:0070936 ISO
Subcellular Localization
    ubiquitin ligase complex GO:0000151 ISO
    nucleus GO:0005634 IBA
    protein-containing complex GO:0032991 ISO
 Experiment description of studies that identified Ube2d2a in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Ube2d2a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Dapk3  
Affinity Capture-Western Mus musculus
2 Rnf25  
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
Two-hybrid Mus musculus
3 Mapt  
Co-fractionation Mus musculus
4 Ube2v1 66589
Co-fractionation Mus musculus
5 Rbx1  
Reconstituted Complex Mus musculus
6 Ube2d1  
Co-fractionation Mus musculus
7 Ube2n 93765
Co-fractionation Mus musculus
8 NEDD4L 23327
Reconstituted Complex Homo sapiens
9 Trim17  
Reconstituted Complex Mus musculus
View the network image/svg+xml
 Pathways in which Ube2d2a is involved
PathwayEvidenceSource
Adaptive Immune System IEA Reactome
Antigen processing: Ubiquitination & Proteasome degradation IEA Reactome
Autophagy IEA Reactome
C-type lectin receptors (CLRs) IEA Reactome
Cellular response to hypoxia IEA Reactome
Cellular responses to stimuli IEA Reactome
Cellular responses to stress IEA Reactome
Class I MHC mediated antigen processing & presentation IEA Reactome
CLEC7A (Dectin-1) signaling IEA Reactome
Cytokine Signaling in Immune system IEA Reactome
Death Receptor Signaling IEA Reactome
Downstream TCR signaling IEA Reactome
E3 ubiquitin ligases ubiquitinate target proteins IEA Reactome
Fc epsilon receptor (FCERI) signaling IEA Reactome
FCERI mediated NF-kB activation IEA Reactome
IKK complex recruitment mediated by RIP1 IEA Reactome
Immune System IEA Reactome
Inactivation of CSF3 (G-CSF) signaling IEA Reactome
Innate Immune System IEA Reactome
Macroautophagy IEA Reactome
Metabolism of proteins IEA Reactome
Mitophagy IEA Reactome
MyD88-independent TLR4 cascade IEA Reactome
Neddylation IEA Reactome
Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha IEA Reactome
Peroxisomal protein import IEA Reactome
PINK1-PRKN Mediated Mitophagy IEA Reactome
Post-translational protein modification IEA Reactome
Protein localization IEA Reactome
Protein ubiquitination IEA Reactome
Regulation of TNFR1 signaling IEA Reactome
Selective autophagy IEA Reactome
Signal Transduction IEA Reactome
Signaling by CSF3 (G-CSF) IEA Reactome
Synthesis of active ubiquitin: roles of E1 and E2 enzymes IEA Reactome
TCR signaling IEA Reactome
TNF signaling IEA Reactome
Toll Like Receptor 4 (TLR4) Cascade IEA Reactome
Toll-like Receptor Cascades IEA Reactome
TRIF (TICAM1)-mediated TLR4 signaling IEA Reactome





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