Gene description for ACKR3
Gene name atypical chemokine receptor 3
Gene symbol ACKR3
Other names/aliases CMKOR1
CXC-R7
CXCR-7
CXCR7
GPR159
RDC-1
RDC1
Species Homo sapiens
 Database cross references - ACKR3
ExoCarta ExoCarta_57007
Vesiclepedia VP_57007
Entrez Gene 57007
HGNC 23692
MIM 610376
UniProt P25106  
 ACKR3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for ACKR3
Molecular Function
    scavenger receptor activity GO:0005044 IMP
    protein binding GO:0005515 IPI
    coreceptor activity GO:0015026 IEA
    C-C chemokine receptor activity GO:0016493 IBA
    C-X-C chemokine receptor activity GO:0016494 IMP
    C-C chemokine binding GO:0019957 IBA
    C-X-C chemokine binding GO:0019958 IMP
Biological Process
    angiogenesis GO:0001525 IEA
    vasculogenesis GO:0001570 IEA
    immune response GO:0006955 IBA
    cell adhesion GO:0007155 IEA
    positive regulation of cytosolic calcium ion concentration GO:0007204 IBA
    negative regulation of cell population proliferation GO:0008285 IEA
    calcium-mediated signaling GO:0019722 IBA
    oculomotor nerve development GO:0021557 IMP
    receptor internalization GO:0031623 IMP
    cell chemotaxis GO:0060326 IBA
    chemokine-mediated signaling pathway GO:0070098 IMP
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IMP
    negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage GO:1902230 IMP
    positive regulation of mesenchymal stem cell migration GO:1905322 IEA
Subcellular Localization
    endosome GO:0005768 IDA
    early endosome GO:0005769 IEA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    clathrin-coated pit GO:0005905 IDA
    external side of plasma membrane GO:0009897 IBA
    cell surface GO:0009986 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
    recycling endosome GO:0055037 IEA
 Experiment description of studies that identified ACKR3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ACKR3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ESPL1  
Affinity Capture-MS Homo sapiens
2 ATP13A2  
Two-hybrid Homo sapiens
3 HECTD2  
Affinity Capture-MS Homo sapiens
4 ATP5H 10476
Affinity Capture-MS Homo sapiens
5 ATP5A1 498
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 GJB2  
Affinity Capture-MS Homo sapiens
7 ATP5D 513
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 MRPL4 51073
Affinity Capture-MS Homo sapiens
9 CXCR4 7852
Affinity Capture-Western Homo sapiens
10 ATP5O 539
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 ATP5B 506
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 UBR7  
Affinity Capture-MS Homo sapiens
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