Gene description for RAB25
Gene name RAB25, member RAS oncogene family
Gene symbol RAB25
Other names/aliases CATX-8
RAB11C
Species Homo sapiens
 Database cross references - RAB25
ExoCarta ExoCarta_57111
Vesiclepedia VP_57111
Entrez Gene 57111
HGNC 18238
MIM 612942
UniProt P57735  
 RAB25 identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 25890246    
Colorectal cancer cells 34887515    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for RAB25
Molecular Function
    GTPase activity GO:0003924 IBA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IBA
    myosin V binding GO:0031489 IPI
Biological Process
    epithelial cell morphogenesis GO:0003382 ISS
    exocytosis GO:0006887 IBA
    positive regulation of cell population proliferation GO:0008284 IDA
    positive regulation of epithelial cell migration GO:0010634 IMP
    protein transport GO:0015031 IEA
    pseudopodium organization GO:0031268 IDA
    regulation of vesicle-mediated transport GO:0060627 IMP
Subcellular Localization
    Golgi apparatus GO:0005794 IBA
    plasma membrane GO:0005886 IDA
    pseudopodium GO:0031143 IDA
    pseudopodium membrane GO:0031260 IEA
    cytoplasmic vesicle GO:0031410 IDA
    recycling endosome GO:0055037 IBA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified RAB25 in sEVs
1
Experiment ID 286
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
3
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for RAB25
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 OPTN 10133
Co-crystal Structure Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
2 AP2S1 1175
Affinity Capture-MS Homo sapiens
3 ARSA 410
Affinity Capture-MS Homo sapiens
4 KPTN  
Affinity Capture-MS Homo sapiens
5 PPP2R2B 5521
Affinity Capture-MS Homo sapiens
6 PKIG  
Affinity Capture-MS Homo sapiens
7 RAB11FIP3  
Two-hybrid Homo sapiens
8 RAB11B 9230
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 FAM60A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 COMMD5 28991
Affinity Capture-MS Homo sapiens
11 MGARP  
Affinity Capture-MS Homo sapiens
12 SH3BP5L  
Two-hybrid Homo sapiens
13 CDK15  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 ST6GALNAC6 30815
Affinity Capture-MS Homo sapiens
15 SOX2  
Affinity Capture-MS Homo sapiens
16 RAB11FIP1 80223
Two-hybrid Homo sapiens
17 SRRT 51593
Affinity Capture-MS Homo sapiens
18 CDH1 999
Negative Genetic Homo sapiens
19 MYO5B 4645
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
20 RAB11A 8766
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 GOT1 2805
Affinity Capture-MS Homo sapiens
22 RAB11FIP2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
23 PDE4DIP 9659
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 HUS1B  
Affinity Capture-MS Homo sapiens
25 ENTPD2 954
Affinity Capture-MS Homo sapiens
26 OR2A4 79541
Affinity Capture-MS Homo sapiens
27 RAB11FIP5 26056
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
28 STX17 55014
Affinity Capture-MS Homo sapiens
29 ATP4A 495
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which RAB25 is involved
PathwayEvidenceSource
Metabolism of proteins TAS Reactome
Post-translational protein modification TAS Reactome
RAB geranylgeranylation TAS Reactome





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