Gene description for Ncstn
Gene name nicastrin
Gene symbol Ncstn
Other names/aliases 9430068N19Rik
AA727311
Aph2
D1Dau13e
Kiaa0253
Nct
mKIAA0253
Species Mus musculus
 Database cross references - Ncstn
ExoCarta ExoCarta_59287
Vesiclepedia VP_59287
Entrez Gene 59287
UniProt P57716  
 Ncstn identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ncstn
Molecular Function
    endopeptidase activity GO:0004175 IDA
    endopeptidase activity GO:0004175 IMP
    protein binding GO:0005515 IPI
    peptidase activity GO:0008233 ISO
    protein-macromolecule adaptor activity GO:0030674 IEA
    protein-macromolecule adaptor activity GO:0030674 ISO
    aspartic endopeptidase activity, intramembrane cleaving GO:0042500 IGI
    ATPase binding GO:0051117 IEA
    ATPase binding GO:0051117 ISO
    growth factor receptor binding GO:0070851 IEA
    growth factor receptor binding GO:0070851 ISO
Biological Process
    myeloid cell homeostasis GO:0002262 IMP
    membrane protein ectodomain proteolysis GO:0006509 ISO
    membrane protein ectodomain proteolysis GO:0006509 ISS
    G protein-coupled dopamine receptor signaling pathway GO:0007212 IMP
    glutamate receptor signaling pathway GO:0007215 IMP
    Notch signaling pathway GO:0007219 IEA
    Notch receptor processing GO:0007220 IBA
    Notch receptor processing GO:0007220 ISO
    Notch receptor processing GO:0007220 NAS
    learning or memory GO:0007611 IMP
    positive regulation of endopeptidase activity GO:0010950 ISO
    protein processing GO:0016485 IBA
    protein processing GO:0016485 ISO
    protein processing GO:0016485 ISS
    cerebellum development GO:0021549 IEA
    central nervous system myelination GO:0022010 IMP
    adult behavior GO:0030534 IMP
    membrane protein intracellular domain proteolysis GO:0031293 ISO
    membrane protein intracellular domain proteolysis GO:0031293 ISS
    amyloid-beta formation GO:0034205 IGI
    amyloid-beta formation GO:0034205 ISO
    amyloid-beta formation GO:0034205 ISS
    T cell proliferation GO:0042098 IMP
    amyloid precursor protein metabolic process GO:0042982 IMP
    amyloid precursor protein metabolic process GO:0042982 ISO
    amyloid precursor protein metabolic process GO:0042982 ISS
    amyloid precursor protein biosynthetic process GO:0042983 IMP
    positive regulation of amyloid precursor protein biosynthetic process GO:0042986 IMP
    amyloid precursor protein catabolic process GO:0042987 ISO
    amyloid precursor protein catabolic process GO:0042987 ISS
    amyloid-beta metabolic process GO:0050435 IMP
    epithelial cell proliferation GO:0050673 IGI
    epithelial cell proliferation GO:0050673 IMP
    neuron apoptotic process GO:0051402 IMP
    cellular response to calcium ion GO:0071277 IMP
    regulation of long-term synaptic potentiation GO:1900271 IMP
    short-term synaptic potentiation GO:1990926 IMP
Subcellular Localization
    Golgi membrane GO:0000139 NAS
    lysosome GO:0005764 IDA
    lysosomal membrane GO:0005765 IEA
    lysosomal membrane GO:0005765 ISO
    early endosome GO:0005769 IEA
    early endosome GO:0005769 ISO
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum GO:0005783 ISS
    endoplasmic reticulum GO:0005783 NAS
    endoplasmic reticulum membrane GO:0005789 NAS
    Golgi apparatus GO:0005794 IDA
    Golgi apparatus GO:0005794 ISO
    Golgi apparatus GO:0005794 ISS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    plasma membrane GO:0005886 TAS
    synaptic vesicle GO:0008021 IDA
    synaptic vesicle GO:0008021 ISO
    membrane GO:0016020 IDA
    membrane GO:0016020 ISO
    membrane GO:0016020 ISS
    cytoplasmic vesicle membrane GO:0030659 IEA
    protein-containing complex GO:0032991 IDA
    protein-containing complex GO:0032991 ISO
    sarcolemma GO:0042383 IEA
    sarcolemma GO:0042383 ISO
    melanosome GO:0042470 IEA
    presynaptic membrane GO:0042734 IEA
    presynaptic membrane GO:0042734 ISO
    gamma-secretase complex GO:0070765 IDA
    gamma-secretase complex GO:0070765 ISO
    gamma-secretase complex GO:0070765 ISS
    synaptic membrane GO:0097060 ISO
 Experiment description of studies that identified Ncstn in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ncstn
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SLC25A13 10165
Affinity Capture-MS Homo sapiens
2 PRKAA1 5562
Affinity Capture-MS Homo sapiens
3 Rer1  
Co-fractionation Mus musculus
4 Herpud1  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
5 SPTLC1 10558
Affinity Capture-MS Homo sapiens
6 MAD2L1 4085
Affinity Capture-MS Homo sapiens
7 Aph1b  
Affinity Capture-MS Mus musculus
8 CANX 821
Affinity Capture-MS Homo sapiens
9 MCAT 27349
Affinity Capture-MS Homo sapiens
10 NDUFA13 51079
Affinity Capture-MS Homo sapiens
11 Npr1  
Co-fractionation Mus musculus
12 LMNB1 4001
Affinity Capture-MS Homo sapiens
13 ASF1B  
Affinity Capture-MS Homo sapiens
14 BSG 682
Affinity Capture-MS Homo sapiens
15 PRR12 57479
Affinity Capture-MS Homo sapiens
16 MBD4  
Affinity Capture-MS Homo sapiens
17 RIF1  
Affinity Capture-MS Homo sapiens
18 Psen1  
Affinity Capture-Western Mus musculus
19 NDUFB5 4711
Affinity Capture-MS Homo sapiens
20 SLC1A5 6510
Affinity Capture-MS Homo sapiens
21 ERLEC1 27248
Affinity Capture-MS Homo sapiens
22 ARCN1 372
Affinity Capture-MS Homo sapiens
23 TRMT6 51605
Affinity Capture-MS Homo sapiens
24 SLC35F6 54978
Affinity Capture-MS Homo sapiens
25 COPA 1314
Affinity Capture-MS Homo sapiens
26 GGCX 2677
Affinity Capture-MS Homo sapiens
27 WLS 79971
Affinity Capture-MS Homo sapiens
28 ATP5J2 9551
Affinity Capture-MS Homo sapiens
29 KIAA2013 90231
Affinity Capture-MS Homo sapiens
30 MCU 90550
Affinity Capture-MS Homo sapiens
31 Aph1a 226548
Affinity Capture-MS Mus musculus
32 PSEN1 5663
Affinity Capture-MS Homo sapiens
33 TIMM23 100287932
Affinity Capture-MS Homo sapiens
34 STT3B 201595
Affinity Capture-MS Homo sapiens
35 NOB1  
Affinity Capture-MS Homo sapiens
36 OS9 10956
Affinity Capture-MS Homo sapiens
37 TCEB1 6921
Affinity Capture-MS Homo sapiens
38 DERL1 79139
Affinity Capture-MS Homo sapiens
39 Apbb2  
Co-fractionation Mus musculus
40 Syvn1  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
41 SCAMP3 10067
Affinity Capture-MS Homo sapiens
42 Vcp 269523
Affinity Capture-Western Mus musculus
43 Psen2  
Affinity Capture-Western Mus musculus
44 Fancd2  
Affinity Capture-MS Mus musculus
45 MRPL2 51069
Affinity Capture-MS Homo sapiens
46 GNL3L 54552
Affinity Capture-MS Homo sapiens
47 TATDN3  
Affinity Capture-MS Homo sapiens
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