Gene description for Pfkp
Gene name phosphofructokinase, platelet
Gene symbol Pfkp
Other names/aliases ATP-PFK
PFK-C
PFK-P
Species Rattus norvegicus
 Database cross references - Pfkp
ExoCarta ExoCarta_60416
Vesiclepedia VP_60416
Entrez Gene 60416
UniProt P47860  
 Pfkp identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Pfkp
Molecular Function
    6-phosphofructokinase activity GO:0003872 IBA
    6-phosphofructokinase activity GO:0003872 IDA
    6-phosphofructokinase activity GO:0003872 IEA
    6-phosphofructokinase activity GO:0003872 ISS
    ATP binding GO:0005524 IDA
    ATP binding GO:0005524 IEA
    identical protein binding GO:0042802 IPI
    identical protein binding GO:0042802 ISO
    protein-containing complex binding GO:0044877 IEA
    protein-containing complex binding GO:0044877 ISO
    metal ion binding GO:0046872 IEA
    fructose-6-phosphate binding GO:0070095 IBA
    fructose-6-phosphate binding GO:0070095 IDA
Biological Process
    fructose 6-phosphate metabolic process GO:0006002 IBA
    fructose 6-phosphate metabolic process GO:0006002 IDA
    fructose 6-phosphate metabolic process GO:0006002 IEA
    glycolytic process GO:0006096 IDA
    fructose 1,6-bisphosphate metabolic process GO:0030388 IBA
    fructose 1,6-bisphosphate metabolic process GO:0030388 IDA
    glycolytic process through fructose-6-phosphate GO:0061615 IEA
    canonical glycolysis GO:0061621 IBA
    cellular response to leukemia inhibitory factor GO:1990830 IEA
    cellular response to leukemia inhibitory factor GO:1990830 ISO
Subcellular Localization
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    6-phosphofructokinase complex GO:0005945 IBA
    membrane GO:0016020 IBA
 Experiment description of studies that identified Pfkp in sEVs
1
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Pfkp
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DYNLL1 8655
Affinity Capture-MS Homo sapiens
2 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml
 Pathways in which Pfkp is involved
PathwayEvidenceSource
Glucose metabolism IEA Reactome
Glycolysis IEA Reactome
Metabolism IEA Reactome
Metabolism of carbohydrates IEA Reactome





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