Gene description for NAA35
Gene name N(alpha)-acetyltransferase 35, NatC auxiliary subunit
Gene symbol NAA35
Other names/aliases EGAP
MAK10
MAK10P
bA379P1.1
Species Homo sapiens
 Database cross references - NAA35
ExoCarta ExoCarta_60560
Vesiclepedia VP_60560
Entrez Gene 60560
HGNC 24340
UniProt Q5VZE5  
 NAA35 identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for NAA35
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    negative regulation of apoptotic process GO:0043066 IBA
    negative regulation of apoptotic process GO:0043066 IMP
    smooth muscle cell proliferation GO:0048659 ISS
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISS
    cytoplasm GO:0005737 NAS
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IDA
    NatC complex GO:0031417 IBA
    NatC complex GO:0031417 IDA
    NatC complex GO:0031417 IPI
 Experiment description of studies that identified NAA35 in sEVs
1
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NAA35
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FGL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 CALR 811
Co-fractionation Homo sapiens
3 ANKZF1 55139
Co-fractionation Homo sapiens
4 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
5 PSMB10 5699
Affinity Capture-MS Homo sapiens
6 DUSP19  
Affinity Capture-MS Homo sapiens
7 NAA38  
Affinity Capture-MS Homo sapiens
8 DNAJC2 27000
Proximity Label-MS Homo sapiens
9 CD2BP2 10421
Affinity Capture-MS Homo sapiens
10 BTF3 689
Affinity Capture-MS Homo sapiens
11 SMNDC1  
Affinity Capture-MS Homo sapiens
12 TRIM7  
Two-hybrid Homo sapiens
13 C1orf35  
Affinity Capture-MS Homo sapiens
14 COX8A  
Proximity Label-MS Homo sapiens
15 RPS3 6188
Co-fractionation Homo sapiens
16 FASN 2194
Negative Genetic Homo sapiens
17 NAA30 122830
Affinity Capture-MS Homo sapiens
18 RPA3 6119
Proximity Label-MS Homo sapiens
19 NTRK1 4914
Affinity Capture-MS Homo sapiens
20 RPL31 6160
Proximity Label-MS Homo sapiens
21 ANK3  
Proximity Label-MS Homo sapiens
22 FAM133A 286499
Affinity Capture-MS Homo sapiens
23 PAGE1  
Affinity Capture-MS Homo sapiens
24 BRD4 23476
Affinity Capture-MS Homo sapiens
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