Gene description for CEACAM1
Gene name carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein)
Gene symbol CEACAM1
Other names/aliases BGP
BGP1
BGPI
Species Homo sapiens
 Database cross references - CEACAM1
ExoCarta ExoCarta_634
Vesiclepedia VP_634
Entrez Gene 634
HGNC 1814
MIM 109770
UniProt P13688  
 CEACAM1 identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 25890246    
Colorectal cancer cells 34887515    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Melanoma cells 25950383    
Melanoma cells 25950383    
Pancreatic duct fluid 29339034    
Pancreatic duct fluid 29339034    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Saliva 19199708    
 Gene ontology annotations for CEACAM1
Molecular Function
    actin binding GO:0003779 IPI
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 ISS
    bile acid transmembrane transporter activity GO:0015125 ISS
    kinase binding GO:0019900 IPI
    protein phosphatase binding GO:0019903 IPI
    filamin binding GO:0031005 IPI
    identical protein binding GO:0042802 IDA
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 ISS
    protein dimerization activity GO:0046983 ISS
    protein tyrosine kinase binding GO:1990782 IBA
    protein tyrosine kinase binding GO:1990782 IPI
Biological Process
    angiogenesis GO:0001525 NAS
    regulation of cell growth GO:0001558 ISS
    blood vessel development GO:0001568 ISS
    negative regulation of T cell mediated cytotoxicity GO:0001915 IDA
    regulation of immune system process GO:0002682 IBA
    negative regulation of natural killer cell mediated cytotoxicity directed against tumor cell target GO:0002859 IMP
    negative regulation of protein kinase activity GO:0006469 ISS
    cell adhesion GO:0007155 ISS
    homophilic cell adhesion via plasma membrane adhesion molecules GO:0007156 ISS
    homophilic cell adhesion via plasma membrane adhesion molecules GO:0007156 NAS
    signal transduction GO:0007165 IBA
    integrin-mediated signaling pathway GO:0007229 NAS
    regulation of endothelial cell migration GO:0010594 ISS
    bile acid and bile salt transport GO:0015721 ISS
    cell migration GO:0016477 NAS
    regulation of cell migration GO:0030334 IDA
    negative regulation of granulocyte differentiation GO:0030853 ISS
    negative regulation of interleukin-1 production GO:0032692 ISS
    cellular response to insulin stimulus GO:0032869 ISS
    common myeloid progenitor cell proliferation GO:0035726 ISS
    insulin receptor internalization GO:0038016 ISS
    granulocyte colony-stimulating factor signaling pathway GO:0038158 ISS
    regulation of epidermal growth factor receptor signaling pathway GO:0042058 ISS
    negative regulation of vascular permeability GO:0043116 ISS
    negative regulation of cytotoxic T cell degranulation GO:0043318 IDA
    wound healing, spreading of cells GO:0044319 IDA
    regulation of endothelial cell differentiation GO:0045601 ISS
    negative regulation of fatty acid biosynthetic process GO:0045717 ISS
    negative regulation of T cell receptor signaling pathway GO:0050860 IDA
    negative regulation of lipid biosynthetic process GO:0051055 ISS
    regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051896 ISS
    regulation of blood vessel remodeling GO:0060312 ISS
    regulation of ERK1 and ERK2 cascade GO:0070372 ISS
    negative regulation of platelet aggregation GO:0090331 ISS
    cell-cell adhesion via plasma-membrane adhesion molecules GO:0098742 ISS
    insulin catabolic process GO:1901143 ISS
    regulation of homophilic cell adhesion GO:1903385 ISS
    regulation of sprouting angiogenesis GO:1903670 ISS
    negative regulation of hepatocyte proliferation GO:2000346 ISS
    positive regulation of vasculogenesis GO:2001214 ISS
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 NAS
    plasma membrane GO:0005886 TAS
    cell-cell junction GO:0005911 IDA
    adherens junction GO:0005912 ISS
    basal plasma membrane GO:0009925 ISS
    cell surface GO:0009986 IBA
    cell surface GO:0009986 IDA
    cell surface GO:0009986 ISS
    membrane GO:0016020 IDA
    apical plasma membrane GO:0016324 IDA
    apical plasma membrane GO:0016324 ISS
    lateral plasma membrane GO:0016328 ISS
    cell junction GO:0030054 IDA
    cell junction GO:0030054 ISS
    transport vesicle membrane GO:0030658 IEA
    microvillus membrane GO:0031528 IEA
    specific granule membrane GO:0035579 TAS
    T cell receptor complex GO:0042101 IDA
    extracellular exosome GO:0070062 HDA
    tertiary granule membrane GO:0070821 TAS
 Experiment description of studies that identified CEACAM1 in sEVs
1
Experiment ID 286
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
3
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 254
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name MNT-1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 257
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 622
MISEV standards
✔
EM
Biophysical techniques
Enriched markers
✔
GOLGA2|CYC1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 29339034    
Organism Homo sapiens
Experiment description Extracellular matrix proteins and carcinoembryonic antigen-related cell adhesion molecules characterize pancreatic duct fluid exosomes in patients with pancreatic cancer
Authors "Zheng J, Hernandez JM, Doussot A, Bojmar L, Zambirinis CP, Costa-Silva B, van Beek EJAH, Mark MT, Molina H, Askan G, Basturk O, Gonen M, Kingham TP, Allen PJ, D'Angelica MI, DeMatteo RP, Lyden D, Jarnagin WR."
Journal name HPB
Publication year 2018
Sample Pancreatic duct fluid
Sample name From patients with Pancreatic ductal adenocarcinoma
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 625
MISEV standards
✘ Biophysical techniques
Enriched markers
✔
GOLGA2|CYC1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 29339034    
Organism Homo sapiens
Experiment description Extracellular matrix proteins and carcinoembryonic antigen-related cell adhesion molecules characterize pancreatic duct fluid exosomes in patients with pancreatic cancer
Authors "Zheng J, Hernandez JM, Doussot A, Bojmar L, Zambirinis CP, Costa-Silva B, van Beek EJAH, Mark MT, Molina H, Askan G, Basturk O, Gonen M, Kingham TP, Allen PJ, D'Angelica MI, DeMatteo RP, Lyden D, Jarnagin WR."
Journal name HPB
Publication year 2018
Sample Pancreatic duct fluid
Sample name From patients with benign pancreatic disease
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for CEACAM1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CEP57  
Affinity Capture-MS Homo sapiens
2 ADRB2  
PCA Homo sapiens
Affinity Capture-Luminescence Homo sapiens
3 PTPN11 5781
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
4 ANXA2 302
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
5 CEACAM8 1088
Reconstituted Complex Homo sapiens
6 SHC1 6464
Affinity Capture-Western Homo sapiens
7 PXN 5829
Affinity Capture-Western Homo sapiens
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