Gene description for RBSN
Gene name rabenosyn, RAB effector
Gene symbol RBSN
Other names/aliases Rabenosyn-5
ZFYVE20
Species Homo sapiens
 Database cross references - RBSN
ExoCarta ExoCarta_64145
Vesiclepedia VP_64145
Entrez Gene 64145
HGNC 20759
MIM 609511
UniProt Q9H1K0  
 RBSN identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for RBSN
Molecular Function
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 NAS
    small GTPase binding GO:0031267 IEA
    phosphatidylinositol-3-phosphate binding GO:0032266 IDA
Biological Process
    protein transport GO:0015031 IEA
    endosomal transport GO:0016197 NAS
    early endosome to Golgi transport GO:0034498 IMP
    Golgi to lysosome transport GO:0090160 IMP
    regulation of Golgi organization GO:1903358 IMP
Subcellular Localization
    endosome GO:0005768 NAS
    early endosome GO:0005769 IDA
    cytosol GO:0005829 IEA
    plasma membrane GO:0005886 IEA
    endosome membrane GO:0010008 TAS
    early endosome membrane GO:0031901 IEA
    intracellular membrane-bounded organelle GO:0043231 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified RBSN in sEVs
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for RBSN
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RAB22A 57403
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
2 DISC1 27185
Two-hybrid Homo sapiens
3 HOOK1  
Proximity Label-MS Homo sapiens
4 MAGEA3  
Affinity Capture-MS Homo sapiens
5 EHD1 10938
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 AVIL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 TP53 7157
Affinity Capture-MS Homo sapiens
8 EHD4 30844
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 HSPA8 3312
Affinity Capture-Western Homo sapiens
10 ATG9A 79065
Proximity Label-MS Homo sapiens
11 EHD3 30845
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 PDHA1 5160
Affinity Capture-MS Homo sapiens
13 LMX1B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 IFFO1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 LAMP2 3920
Proximity Label-MS Homo sapiens
16 RAB14 51552
Reconstituted Complex Homo sapiens
17 PTK2 5747
Affinity Capture-Western Homo sapiens
18 MYH1  
Affinity Capture-MS Homo sapiens
19 EHD2 30846
Two-hybrid Homo sapiens
20 RAB4A 5867
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Two-hybrid Homo sapiens
21 RAB5A 5868
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Proximity Label-MS Homo sapiens
Far Western Homo sapiens
Reconstituted Complex Homo sapiens
22 Immt  
Affinity Capture-MS Mus musculus
23 ACACA 31
Negative Genetic Homo sapiens
24 DTNBP1  
Two-hybrid Homo sapiens
25 Vps45 64516
Two-hybrid Rattus norvegicus
Reconstituted Complex Rattus norvegicus
26 MUSK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 ANKFY1 51479
Proximity Label-MS Homo sapiens
28 RPA3 6119
Proximity Label-MS Homo sapiens
29 VPS45 11311
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 RAB24 53917
Reconstituted Complex Homo sapiens
31 RAB7A 7879
Proximity Label-MS Homo sapiens
32 PCDHA4 56144
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 EPHA2 1969
Proximity Label-MS Homo sapiens
34 RPA4  
Proximity Label-MS Homo sapiens
35 RAB5C 5878
Reconstituted Complex Homo sapiens
Proximity Label-MS Homo sapiens
36 MYH7 4625
Affinity Capture-MS Homo sapiens
37 AGTR1  
Two-hybrid Homo sapiens
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