Gene description for Map1lc3b
Gene name microtubule-associated protein 1 light chain 3 beta
Gene symbol Map1lc3b
Other names/aliases Map1lc3
Mpl3
zbs559
Species Rattus norvegicus
 Database cross references - Map1lc3b
ExoCarta ExoCarta_64862
Vesiclepedia VP_64862
Entrez Gene 64862
UniProt Q62625  
 Map1lc3b identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Map1lc3b
Molecular Function
    microtubule binding GO:0008017 IBA
    microtubule binding GO:0008017 IDA
    phosphatidylethanolamine binding GO:0008429 IBA
    phosphatidylethanolamine binding GO:0008429 ISO
    tubulin binding GO:0015631 IDA
    protein domain specific binding GO:0019904 IPI
    ubiquitin protein ligase binding GO:0031625 IBA
    ubiquitin protein ligase binding GO:0031625 ISO
    ceramide binding GO:0097001 ISO
Biological Process
    autophagosome assembly GO:0000045 IBA
    autophagosome assembly GO:0000045 ISO
    mitophagy GO:0000423 IBA
    mitophagy GO:0000423 ISO
    mitophagy GO:0000423 ISS
    autophagy GO:0006914 IEA
    autophagy GO:0006914 ISO
    cellular response to nitrogen starvation GO:0006995 IBA
    cellular response to starvation GO:0009267 ISO
    cellular response to starvation GO:0009267 ISS
    macroautophagy GO:0016236 ISO
    establishment of localization in cell GO:0051649 ISO
    mucus secretion GO:0070254 ISO
    positive regulation of mucus secretion GO:0070257 ISO
    autophagosome maturation GO:0097352 IBA
    autophagosome maturation GO:0097352 ISO
Subcellular Localization
    autophagosome membrane GO:0000421 IBA
    autophagosome membrane GO:0000421 IDA
    autophagosome membrane GO:0000421 IEA
    autophagosome membrane GO:0000421 ISO
    mitochondrion GO:0005739 ISO
    autophagosome GO:0005776 ISO
    microtubule GO:0005874 IEA
    microtubule associated complex GO:0005875 IDA
    axoneme GO:0005930 ISO
    endomembrane system GO:0012505 IEA
    membrane GO:0016020 ISO
    axon GO:0030424 IDA
    dendrite GO:0030425 IDA
    cytoplasmic vesicle GO:0031410 IEA
    cytoplasmic vesicle GO:0031410 ISO
    cytoplasmic vesicle GO:0031410 ISS
    mitochondrial membrane GO:0031966 IEA
    neuronal cell body GO:0043025 IDA
    intracellular membrane-bounded organelle GO:0043231 ISO
    autolysosome GO:0044754 ISO
    presynapse GO:0098793 IDA
 Experiment description of studies that identified Map1lc3b in sEVs
1
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 99
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Map1lc3b
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DYNLL1 8655
Affinity Capture-MS Homo sapiens
2 Snca  
Affinity Capture-MS Rattus norvegicus
3 Sqstm1  
FRET Mus musculus
Co-localization Mus musculus
4 Tuba1a 64158
Reconstituted Complex Rattus norvegicus
View the network image/svg+xml



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