Gene description for ANXA8
Gene name annexin A8
Gene symbol ANXA8
Other names/aliases ANX8
Species Homo sapiens
 Database cross references - ANXA8
ExoCarta ExoCarta_653145
Vesiclepedia VP_653145
Entrez Gene 653145
HGNC 546
MIM 602396
UniProt P13928   Q5T2P8  
 ANXA8 identified in sEVs derived from the following tissue/cell type
Bladder cancer cells 20224111    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for ANXA8
Molecular Function
    phosphatidylserine binding GO:0001786 IBA
    calcium ion binding GO:0005509 IDA
    protein binding GO:0005515 IPI
    calcium-dependent phospholipid binding GO:0005544 IBA
    calcium-dependent phospholipid binding GO:0005544 IDA
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 IDA
    phosphatidylinositol-3,4,5-trisphosphate binding GO:0005547 IDA
    phosphatidylinositol-3,4-bisphosphate binding GO:0043325 IDA
    actin filament binding GO:0051015 IDA
Biological Process
    endosome organization GO:0007032 IBA
    endosome organization GO:0007032 IMP
    blood coagulation GO:0007596 IEA
    endosomal transport GO:0016197 IBA
    endosomal transport GO:0016197 IMP
    negative regulation of serine-type endopeptidase activity GO:1900004 IDA
    negative regulation of phospholipase A2 activity GO:1900138 IDA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IDA
    vesicle membrane GO:0012506 IBA
    late endosome membrane GO:0031902 IDA
    sarcolemma GO:0042383 IBA
    collagen-containing extracellular matrix GO:0062023 HDA
 Experiment description of studies that identified ANXA8 in sEVs
1
Experiment ID 76
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors "Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A."
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
2
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ANXA8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CERS3  
Affinity Capture-MS Homo sapiens
2 IFNA4  
Affinity Capture-MS Homo sapiens
3 Cdk1 12534
Affinity Capture-MS Mus musculus
4 KIF3A 11127
Affinity Capture-MS Homo sapiens
5 C18orf21  
Affinity Capture-MS Homo sapiens
6 AGPAT1 10554
Affinity Capture-MS Homo sapiens
7 NRSN1  
Affinity Capture-MS Homo sapiens
8 DDX19B 11269
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 SH2D3A  
Affinity Capture-MS Homo sapiens
10 TBC1D22B  
Affinity Capture-MS Homo sapiens
11 HDAC9  
Affinity Capture-MS Homo sapiens
12 PCP4  
Affinity Capture-MS Homo sapiens
13 C1orf35  
Affinity Capture-MS Homo sapiens
14 APP 351
Reconstituted Complex Homo sapiens
15 ST6GALNAC6 30815
Affinity Capture-MS Homo sapiens
16 TREX2  
Affinity Capture-MS Homo sapiens
17 PTDSS1 9791
Affinity Capture-MS Homo sapiens
18 FUNDC2 65991
Affinity Capture-MS Homo sapiens
19 ST7  
Affinity Capture-MS Homo sapiens
20 SOX2  
Affinity Capture-MS Homo sapiens
21 FNDC5  
Affinity Capture-MS Homo sapiens
22 KLHL11  
Affinity Capture-MS Homo sapiens
23 RNF115  
Affinity Capture-MS Homo sapiens
24 SRRT 51593
Affinity Capture-MS Homo sapiens
25 ERCC3  
Affinity Capture-MS Homo sapiens
26 NAMPT 10135
Co-fractionation Homo sapiens
27 MBNL1 4154
Affinity Capture-MS Homo sapiens
28 ACTA1 58
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 SLC25A6 293
Affinity Capture-MS Homo sapiens
30 ECHDC3 79746
Co-fractionation Homo sapiens
31 SIK1  
Affinity Capture-MS Homo sapiens
32 SARAF  
Affinity Capture-MS Homo sapiens
33 PINK1  
Affinity Capture-MS Homo sapiens
34 GOT1 2805
Affinity Capture-MS Homo sapiens
35 STK11 6794
Affinity Capture-MS Homo sapiens
36 SSUH2  
Affinity Capture-MS Homo sapiens
37 PDE4DIP 9659
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 MMAB 326625
Co-fractionation Homo sapiens
39 POU3F3  
Affinity Capture-MS Homo sapiens
40 HCN1  
Affinity Capture-MS Homo sapiens
41 UPK1A 11045
Affinity Capture-MS Homo sapiens
42 CCR1  
Affinity Capture-MS Homo sapiens
43 RIPPLY3  
Affinity Capture-MS Homo sapiens
44 DKK2  
Affinity Capture-MS Homo sapiens
45 OR2A4 79541
Affinity Capture-MS Homo sapiens
46 UCHL5 51377
Reconstituted Complex Homo sapiens
47 PHF11 51131
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ANXA8 is involved
No pathways found





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