Gene description for SLC6A8
Gene name solute carrier family 6 (neurotransmitter transporter), member 8
Gene symbol SLC6A8
Other names/aliases CCDS1
CRT
CRTR
CT1
CTR5
Species Homo sapiens
 Database cross references - SLC6A8
ExoCarta ExoCarta_6535
Vesiclepedia VP_6535
Entrez Gene 6535
HGNC 11055
MIM 300036
UniProt P48029  
 SLC6A8 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Hepatocellular carcinoma cells 26054723    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Neuroblastoma cells 25944692    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for SLC6A8
Molecular Function
    creatine transmembrane transporter activity GO:0005308 NAS
    creatine:sodium symporter activity GO:0005309 IDA
    creatine:sodium symporter activity GO:0005309 IMP
    creatine:sodium symporter activity GO:0005309 TAS
    gamma-aminobutyric acid:sodium:chloride symporter activity GO:0005332 IBA
Biological Process
    creatine metabolic process GO:0006600 TAS
    neurotransmitter transport GO:0006836 IEA
    amino acid transport GO:0006865 IBA
    muscle contraction GO:0006936 TAS
    gamma-aminobutyric acid transport GO:0015812 IEA
    creatine transmembrane transport GO:0015881 IMP
    creatine transmembrane transport GO:0015881 NAS
    sodium ion transmembrane transport GO:0035725 IBA
    nitrogen compound transport GO:0071705 IDA
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IMP
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 TAS
    apical plasma membrane GO:0016324 IDA
 Experiment description of studies that identified SLC6A8 in sEVs
1
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 282
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
6
Experiment ID 283
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
7
Experiment ID 285
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
8
Experiment ID 236
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
9
Experiment ID 255
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 256
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 257
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 258
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
13
Experiment ID 224
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
14
Experiment ID 834
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
15
Experiment ID 835
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for SLC6A8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GRPR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 CANX 821
Affinity Capture-MS Homo sapiens
3 NT5E 4907
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 CDS2 8760
Affinity Capture-MS Homo sapiens
5 GJD3  
Proximity Label-MS Homo sapiens
6 RAB35 11021
Proximity Label-MS Homo sapiens
7 FASN 2194
Negative Genetic Homo sapiens
8 PTGIR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 TMEM136  
Affinity Capture-MS Homo sapiens
10 PTDSS1 9791
Affinity Capture-MS Homo sapiens
11 EBAG9 9166
Proximity Label-MS Homo sapiens
12 SLC31A2  
Affinity Capture-MS Homo sapiens
13 OCLN 100506658
Proximity Label-MS Homo sapiens
14 NTSR1  
Affinity Capture-MS Homo sapiens
15 GPR17 2840
Affinity Capture-MS Homo sapiens
16 METTL7A 25840
Proximity Label-MS Homo sapiens
17 LAMTOR1 55004
Proximity Label-MS Homo sapiens
18 MARCKS 4082
Proximity Label-MS Homo sapiens
19 LAMP3  
Proximity Label-MS Homo sapiens
20 RAB5A 5868
Proximity Label-MS Homo sapiens
21 PANX1 24145
Proximity Label-MS Homo sapiens
22 GJA1 2697
Proximity Label-MS Homo sapiens
23 RAB11A 8766
Proximity Label-MS Homo sapiens
24 LAMP1 3916
Proximity Label-MS Homo sapiens
25 EZR 7430
Proximity Label-MS Homo sapiens
26 SLC26A6 65010
Affinity Capture-MS Homo sapiens
27 BCAP31 10134
Proximity Label-MS Homo sapiens
28 HSD3B7 80270
Proximity Label-MS Homo sapiens
29 LMAN1 3998
Proximity Label-MS Homo sapiens
30 GCGR  
Affinity Capture-MS Homo sapiens
31 HSD17B11 51170
Proximity Label-MS Homo sapiens
32 TPRA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 LAMP2 3920
Proximity Label-MS Homo sapiens
34 COX8A  
Proximity Label-MS Homo sapiens
35 RPN2 6185
Proximity Label-MS Homo sapiens
36 ARF6 382
Proximity Label-MS Homo sapiens
37 CAV1 857
Proximity Label-MS Homo sapiens
38 CXADR 1525
Proximity Label-MS Homo sapiens
39 ERGIC1 57222
Proximity Label-MS Homo sapiens
40 C11orf52 91894
Proximity Label-MS Homo sapiens
41 ILVBL 10994
Affinity Capture-MS Homo sapiens
42 LYN 4067
Proximity Label-MS Homo sapiens
43 OCIAD1 54940
Affinity Capture-MS Homo sapiens
44 MCAM 4162
Proximity Label-MS Homo sapiens
45 RAB4A 5867
Proximity Label-MS Homo sapiens
46 PRKACA 5566
Proximity Label-MS Homo sapiens
47 DNAJC5 80331
Proximity Label-MS Homo sapiens
48 BDKRB1 623
Two-hybrid Homo sapiens
49 EPHA2 1969
Proximity Label-MS Homo sapiens
50 B3GAT1  
Proximity Label-MS Homo sapiens
51 RAB2A 5862
Proximity Label-MS Homo sapiens
52 MLLT4 4301
Proximity Label-MS Homo sapiens
53 SEC62 7095
Proximity Label-MS Homo sapiens
54 DIRAS3  
Proximity Label-MS Homo sapiens
55 DNAJC5B  
Proximity Label-MS Homo sapiens
56 CMTM5  
Affinity Capture-MS Homo sapiens
57 CHRM5  
Two-hybrid Homo sapiens
58 TNFRSF10C  
Affinity Capture-MS Homo sapiens
59 RAB3B 5865
Proximity Label-MS Homo sapiens
60 C5AR1  
Affinity Capture-MS Homo sapiens
61 FPR1  
Affinity Capture-MS Homo sapiens
62 RPN1 6184
Proximity Label-MS Homo sapiens
63 DHFRL1  
Proximity Label-MS Homo sapiens
64 KRAS 3845
Proximity Label-MS Homo sapiens
65 RAB5C 5878
Proximity Label-MS Homo sapiens
66 RHOB 388
Proximity Label-MS Homo sapiens
67 YIPF3 25844
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 CKAP4 10970
Proximity Label-MS Homo sapiens
69 STX4 6810
Proximity Label-MS Homo sapiens
70 SLC22A4 6583
Affinity Capture-MS Homo sapiens
71 AGTR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 ELOVL5 60481
Proximity Label-MS Homo sapiens
73 FPR2  
Affinity Capture-MS Homo sapiens
74 SEC61B 10952
Proximity Label-MS Homo sapiens
75 FLOT1 10211
Proximity Label-MS Homo sapiens
76 STX6 10228
Proximity Label-MS Homo sapiens
77 RAB9A 9367
Proximity Label-MS Homo sapiens
View the network image/svg+xml
 Pathways in which SLC6A8 is involved
PathwayEvidenceSource
Creatine metabolism TAS Reactome
Metabolism TAS Reactome
Metabolism of amino acids and derivatives TAS Reactome





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