Gene description for Clstn1
Gene name calsyntenin 1
Gene symbol Clstn1
Other names/aliases 1810034E21Rik
Cst-1
Cstn1
Species Mus musculus
 Database cross references - Clstn1
ExoCarta ExoCarta_65945
Vesiclepedia VP_65945
Entrez Gene 65945
UniProt Q9EPL2  
 Clstn1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Clstn1
Molecular Function
    amyloid-beta binding GO:0001540 IEA
    amyloid-beta binding GO:0001540 ISO
    calcium ion binding GO:0005509 NAS
    protein binding GO:0005515 IPI
    kinesin binding GO:0019894 IEA
    kinesin binding GO:0019894 ISO
    X11-like protein binding GO:0042988 IEA
    X11-like protein binding GO:0042988 ISO
Biological Process
    regulation of cell growth GO:0001558 IGI
    intracellular calcium ion homeostasis GO:0006874 TAS
    homophilic cell adhesion via plasma membrane adhesion molecules GO:0007156 IEA
    chemical synaptic transmission GO:0007268 NAS
    positive regulation of synaptic transmission GO:0050806 IBA
    positive regulation of synaptic transmission GO:0050806 IGI
    positive regulation of synapse assembly GO:0051965 IBA
    positive regulation of synapse assembly GO:0051965 IGI
    regulation of synapse maturation GO:0090128 IDA
    regulation of synapse maturation GO:0090128 IEP
    regulation of synapse maturation GO:0090128 IMP
    neurotransmitter receptor transport to postsynaptic membrane GO:0098969 IDA
    neurotransmitter receptor transport to postsynaptic membrane GO:0098969 IEP
    neurotransmitter receptor transport to postsynaptic membrane GO:0098969 IMP
    vesicle-mediated transport in synapse GO:0099003 IDA
    vesicle-mediated transport in synapse GO:0099003 IEP
    vesicle-mediated transport in synapse GO:0099003 IMP
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    extracellular region GO:0005576 IDA
    nucleus GO:0005634 IEA
    endoplasmic reticulum membrane GO:0005789 IEA
    cell surface GO:0009986 IBA
    cell surface GO:0009986 IDA
    postsynaptic density GO:0014069 IDA
    membrane GO:0016020 IDA
    neuron projection GO:0043005 IEA
    postsynaptic membrane GO:0045211 IBA
    postsynaptic membrane GO:0045211 IDA
    postsynaptic density membrane GO:0098839 ISO
    postsynaptic endosome GO:0098845 IDA
    spine apparatus membrane GO:0098897 ISO
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IEP
    glutamatergic synapse GO:0098978 IMP
    glutamatergic synapse GO:0098978 ISO
    GABA-ergic synapse GO:0098982 ISO
 Experiment description of studies that identified Clstn1 in sEVs
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Clstn1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kif5a  
Affinity Capture-Western Mus musculus
2 Mcm3ap  
Co-fractionation Mus musculus
3 Klc1  
Two-hybrid Mus musculus
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
4 Prnp 19122
Affinity Capture-MS Mus musculus
5 Klc2  
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
6 KLC1 3831
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which Clstn1 is involved
No pathways found





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