Gene description for Nop56
Gene name NOP56 ribonucleoprotein
Gene symbol Nop56
Other names/aliases 2310044F10Rik
Nol5a
Species Mus musculus
 Database cross references - Nop56
ExoCarta ExoCarta_67134
Vesiclepedia VP_67134
Entrez Gene 67134
UniProt Q9D6Z1  
 Nop56 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
Neural stem cells 25242146    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Nop56
Molecular Function
    RNA binding GO:0003723 IPI
    snoRNA binding GO:0030515 IBA
    snoRNA binding GO:0030515 IEA
    snoRNA binding GO:0030515 ISO
    histone methyltransferase binding GO:1990226 IEA
    histone methyltransferase binding GO:1990226 ISO
Biological Process
    ribosomal small subunit biogenesis GO:0042274 ISO
    ribosomal small subunit biogenesis GO:0042274 ISS
Subcellular Localization
    fibrillar center GO:0001650 IEA
    fibrillar center GO:0001650 ISO
    nucleoplasm GO:0005654 IEA
    sno(s)RNA-containing ribonucleoprotein complex GO:0005732 ISO
    cytoplasm GO:0005737 IEA
    box C/D methylation guide snoRNP complex GO:0031428 IBA
    box C/D methylation guide snoRNP complex GO:0031428 IEA
    small-subunit processome GO:0032040 IBA
    small-subunit processome GO:0032040 ISO
    small-subunit processome GO:0032040 ISS
    pre-snoRNP complex GO:0070761 IEA
    pre-snoRNP complex GO:0070761 ISO
 Experiment description of studies that identified Nop56 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 263
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th1 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Nop56
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PHB 5245
Two-hybrid Homo sapiens
2 Eed  
Affinity Capture-MS Mus musculus
3 Fxr1 14359
Affinity Capture-MS Mus musculus
4 Tnik  
Affinity Capture-MS Mus musculus
5 Atxn1  
Affinity Capture-MS Mus musculus
6 Cebpa  
Affinity Capture-MS Mus musculus
7 Prmt1 15469
Affinity Capture-MS Mus musculus
8 Tfe3  
Affinity Capture-MS Mus musculus
9 JADE1  
Affinity Capture-MS Homo sapiens
10 KRT31 3881
Two-hybrid Homo sapiens
11 Mettl21e  
Affinity Capture-MS Mus musculus
12 Fancd2  
Affinity Capture-MS Mus musculus
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