Gene description for Atp2b1
Gene name ATPase, Ca++ transporting, plasma membrane 1
Gene symbol Atp2b1
Other names/aliases 2810442I22Rik
E130111D10Rik
Pmca1
Species Mus musculus
 Database cross references - Atp2b1
ExoCarta ExoCarta_67972
Vesiclepedia VP_67972
Entrez Gene 67972
UniProt G5E829  
 Atp2b1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Atp2b1
Molecular Function
    P-type calcium transporter activity GO:0005388 IBA
    P-type calcium transporter activity GO:0005388 IEA
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IEA
    ATP binding GO:0005524 IEA
    calcium ion transmembrane transporter activity GO:0015085 ISO
    ATP hydrolysis activity GO:0016887 ISO
    ATP hydrolysis activity GO:0016887 ISS
    PDZ domain binding GO:0030165 ISO
    metal ion binding GO:0046872 IEA
    molecular function inhibitor activity GO:0140678 ISO
Biological Process
    negative regulation of cytokine production GO:0001818 IMP
    regulation of vascular associated smooth muscle contraction GO:0003056 IMP
    calcium ion transport GO:0006816 TAS
    intracellular calcium ion homeostasis GO:0006874 IMP
    regulation of blood pressure GO:0008217 IMP
    positive regulation of bone mineralization GO:0030501 IMP
    regulation of cytosolic calcium ion concentration GO:0051480 IBA
    regulation of cytosolic calcium ion concentration GO:0051480 IMP
    regulation of cytosolic calcium ion concentration GO:0051480 ISO
    negative regulation of cytosolic calcium ion concentration GO:0051481 IMP
    negative regulation of cytosolic calcium ion concentration GO:0051481 ISO
    negative regulation of cytosolic calcium ion concentration GO:0051481 ISS
    positive regulation of calcium ion transport GO:0051928 IMP
    regulation of cellular response to insulin stimulus GO:1900076 ISO
    regulation of cellular response to insulin stimulus GO:1900076 ISS
    calcium ion export GO:1901660 ISO
    calcium ion export across plasma membrane GO:1990034 IEA
    calcium ion export across plasma membrane GO:1990034 ISO
Subcellular Localization
    immunological synapse GO:0001772 IDA
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 TAS
    cytoplasmic side of plasma membrane GO:0009898 ISO
    basolateral plasma membrane GO:0016323 IDA
    basolateral plasma membrane GO:0016323 ISO
    apical plasma membrane GO:0016324 ISO
    synaptic vesicle membrane GO:0030672 IDA
    dendrite membrane GO:0032590 ISO
    dendritic spine membrane GO:0032591 ISO
    neuronal cell body membrane GO:0032809 ISO
    presynaptic membrane GO:0042734 IDA
    presynaptic membrane GO:0042734 IDA
    cell projection GO:0042995 IEA
    intracellular membrane-bounded organelle GO:0043231 IBA
    apical part of cell GO:0045177 ISO
    presynaptic active zone membrane GO:0048787 ISO
    photoreceptor ribbon synapse GO:0098684 IDA
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 ISO
    GABA-ergic synapse GO:0098982 ISO
 Experiment description of studies that identified Atp2b1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Atp2b1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Itpr3  
Affinity Capture-Western Mus musculus
2 Tmem173  
Proximity Label-MS Mus musculus
3 Sstr3  
Proximity Label-MS Mus musculus
4 Kctd13  
Affinity Capture-MS Mus musculus
5 Atp2a2 11938
Affinity Capture-Western Mus musculus
6 Unk  
Affinity Capture-RNA Mus musculus
7 Fancd2  
Affinity Capture-MS Mus musculus
8 Ywhaz 22631
Affinity Capture-MS Mus musculus
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