Gene description for TFR2
Gene name transferrin receptor 2
Gene symbol TFR2
Other names/aliases HFE3
TFRC2
Species Homo sapiens
 Database cross references - TFR2
ExoCarta ExoCarta_7036
Vesiclepedia VP_7036
Entrez Gene 7036
HGNC 11762
MIM 604720
UniProt Q9UP52  
 TFR2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for TFR2
Molecular Function
    transferrin receptor activity GO:0004998 IDA
    transferrin receptor activity GO:0004998 NAS
    protein binding GO:0005515 IPI
    co-receptor binding GO:0039706 IPI
Biological Process
    iron ion transport GO:0006826 NAS
    intracellular iron ion homeostasis GO:0006879 IDA
    intracellular iron ion homeostasis GO:0006879 TAS
    receptor-mediated endocytosis GO:0006898 IGI
    acute-phase response GO:0006953 IEA
    response to iron ion GO:0010039 IMP
    transferrin transport GO:0033572 IGI
    positive regulation of endocytosis GO:0045807 IGI
    positive regulation of transcription by RNA polymerase II GO:0045944 IGI
    multicellular organismal-level iron ion homeostasis GO:0060586 IMP
    cellular response to iron ion GO:0071281 IGI
    positive regulation of peptide hormone secretion GO:0090277 IMP
    endocytic iron import into cell GO:0140298 IBA
    endocytic iron import into cell GO:0140298 IGI
    positive regulation of protein maturation GO:1903319 IGI
Subcellular Localization
    plasma membrane GO:0005886 IGI
    plasma membrane GO:0005886 NAS
    plasma membrane GO:0005886 TAS
    external side of plasma membrane GO:0009897 IBA
    external side of plasma membrane GO:0009897 IGI
    cytoplasmic vesicle GO:0031410 IDA
    HFE-transferrin receptor complex GO:1990712 IDA
 Experiment description of studies that identified TFR2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for TFR2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD81 975
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
2 GALNT12 79695
Affinity Capture-MS Homo sapiens
3 EXT1 2131
Affinity Capture-MS Homo sapiens
4 ATP4A 495
Affinity Capture-MS Homo sapiens
5 POP4 10775
Affinity Capture-MS Homo sapiens
6 TF 7018
Reconstituted Complex Homo sapiens
7 MAD2L2 10459
Affinity Capture-MS Homo sapiens
8 BRD4 23476
Affinity Capture-MS Homo sapiens
9 PFKL 5211
Affinity Capture-MS Homo sapiens
10 LRRFIP1 9208
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 TAF5L  
Affinity Capture-MS Homo sapiens
12 GLG1 2734
Affinity Capture-MS Homo sapiens
13 FBXO6 26270
Affinity Capture-MS Homo sapiens
14 TBC1D2B 23102
Affinity Capture-MS Homo sapiens
15 SEC22A  
Two-hybrid Homo sapiens
16 OLFM4 10562
Two-hybrid Homo sapiens
17 SOCS3 9021
Two-hybrid Homo sapiens
18 PDF  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which TFR2 is involved
PathwayEvidenceSource
Iron uptake and transport TAS Reactome
Transferrin endocytosis and recycling TAS Reactome
Transport of small molecules TAS Reactome





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