Gene description for Dock8
Gene name dedicator of cytokinesis 8
Gene symbol Dock8
Other names/aliases 1200017A24Rik
5830472H07Rik
A130095G14Rik
AI461977
Species Mus musculus
 Database cross references - Dock8
ExoCarta ExoCarta_76088
Vesiclepedia VP_76088
Entrez Gene 76088
UniProt Q8C147  
 Dock8 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Dock8
Molecular Function
    guanyl-nucleotide exchange factor activity GO:0005085 IBA
    guanyl-nucleotide exchange factor activity GO:0005085 IDA
    guanyl-nucleotide exchange factor activity GO:0005085 ISO
    protein binding GO:0005515 IPI
Biological Process
    immunological synapse formation GO:0001771 IMP
    small GTPase-mediated signal transduction GO:0007264 IEA
    regulation of Rho protein signal transduction GO:0035023 IBA
    dendritic cell migration GO:0036336 IGI
    dendritic cell migration GO:0036336 IMP
    dendritic cell migration GO:0036336 IMP
    positive regulation of GTPase activity GO:0043547 ISO
    memory T cell proliferation GO:0061485 ISO
    negative regulation of T cell apoptotic process GO:0070233 IMP
    positive regulation of establishment of T cell polarity GO:1903905 IBA
    positive regulation of establishment of T cell polarity GO:1903905 IMP
    positive regulation of establishment of T cell polarity GO:1903905 ISO
    cellular response to chemokine GO:1990869 IEA
    cellular response to chemokine GO:1990869 ISO
    positive regulation of T cell migration GO:2000406 IBA
    positive regulation of T cell migration GO:2000406 IMP
    positive regulation of T cell migration GO:2000406 ISO
Subcellular Localization
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 ISO
    cell leading edge GO:0031252 IBA
    cell leading edge GO:0031252 IDA
    leading edge membrane GO:0031256 ISO
    lamellipodium membrane GO:0031258 IEA
 Experiment description of studies that identified Dock8 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Dock8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Hba-x  
Co-fractionation Mus musculus
2 Dock6  
Co-fractionation Mus musculus
3 Tmem173  
Proximity Label-MS Mus musculus
4 Kctd13  
Affinity Capture-MS Mus musculus
5 Lrch1  
Co-fractionation Mus musculus
6 Fbxl12  
Co-fractionation Mus musculus
7 Ywhae 22627
Affinity Capture-MS Mus musculus
8 Dock11  
Co-fractionation Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here