Gene description for Aacs
Gene name acetoacetyl-CoA synthetase
Gene symbol Aacs
Other names/aliases 2210408B16Rik
SUR5
Species Mus musculus
 Database cross references - Aacs
ExoCarta ExoCarta_78894
Vesiclepedia VP_78894
Entrez Gene 78894
UniProt Q9D2R0  
 Aacs identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Aacs
Molecular Function
    ATP binding GO:0005524 IEA
    acetoacetate-CoA ligase activity GO:0030729 IBA
    acetoacetate-CoA ligase activity GO:0030729 IEA
    acetoacetate-CoA ligase activity GO:0030729 ISO
    medium-chain fatty acid-CoA ligase activity GO:0031956 ISO
Biological Process
    liver development GO:0001889 ISO
    fatty acid metabolic process GO:0006631 IEA
    response to nutrient GO:0007584 ISO
    response to xenobiotic stimulus GO:0009410 ISO
    response to organic cyclic compound GO:0014070 ISO
    positive regulation of insulin secretion GO:0032024 IBA
    positive regulation of insulin secretion GO:0032024 ISO
    response to oleic acid GO:0034201 ISO
    response to ethanol GO:0045471 ISO
    cellular response to glucose stimulus GO:0071333 ISO
Subcellular Localization
    cytosol GO:0005829 IEA
 Experiment description of studies that identified Aacs in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Aacs
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fasn 14104
Co-fractionation Mus musculus
2 TRAF4 9618
Two-hybrid Homo sapiens
3 Fdps 110196
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Aacs is involved
PathwayEvidenceSource
Ketone body metabolism IEA Reactome
Metabolism IEA Reactome
Metabolism of lipids IEA Reactome
Synthesis of Ketone Bodies IEA Reactome





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