Gene description for Arf4
Gene name ADP-ribosylation factor 4
Gene symbol Arf4
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Arf4
ExoCarta ExoCarta_79120
Vesiclepedia VP_79120
Entrez Gene 79120
UniProt P61751  
 Arf4 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
 Gene ontology annotations for Arf4
Molecular Function
    GTPase activity GO:0003924 IEA
    epidermal growth factor receptor binding GO:0005154 IEA
    epidermal growth factor receptor binding GO:0005154 ISO
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 IEA
    GTP binding GO:0005525 ISO
    NAD+-protein-arginine ADP-ribosyltransferase activity GO:0106274 IEA
    NAD+-protein-arginine ADP-ribosyltransferase activity GO:0106274 ISO
    phospholipase D activator activity GO:1990583 IEA
    phospholipase D activator activity GO:1990583 ISO
Biological Process
    intracellular protein transport GO:0006886 IBA
    retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum GO:0006890 IEA
    retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum GO:0006890 ISO
    epidermal growth factor receptor signaling pathway GO:0007173 IDA
    learning GO:0007612 IEA
    learning GO:0007612 ISO
    protein transport GO:0015031 IEA
    vesicle-mediated transport GO:0016192 IBA
    cell migration GO:0016477 IEA
    cell migration GO:0016477 ISO
    negative regulation of apoptotic process GO:0043066 IEA
    negative regulation of apoptotic process GO:0043066 ISO
    apical protein localization GO:0045176 IEA
    apical protein localization GO:0045176 ISO
    establishment or maintenance of epithelial cell apical/basal polarity GO:0045197 IEA
    establishment or maintenance of epithelial cell apical/basal polarity GO:0045197 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    response to axon injury GO:0048678 IEP
    regulation of synapse organization GO:0050807 ISO
    dendritic spine development GO:0060996 IEA
    dendritic spine development GO:0060996 ISO
    protein localization to cilium GO:0061512 IEA
    protein localization to cilium GO:0061512 ISO
    regulation of postsynapse organization GO:0099175 IEA
    regulation of postsynapse organization GO:0099175 ISO
    regulation of cilium assembly GO:1902017 IEA
    regulation of cilium assembly GO:1902017 ISO
    regulation of cilium assembly GO:1902017 ISS
    regulation of reactive oxygen species metabolic process GO:2000377 IEA
    regulation of reactive oxygen species metabolic process GO:2000377 ISO
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    Golgi membrane GO:0000139 ISO
    Golgi membrane GO:0000139 ISS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    Golgi apparatus GO:0005794 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    ruffle membrane GO:0032587 IEA
    ruffle membrane GO:0032587 ISO
    dendritic spine GO:0043197 IEA
    dendritic spine GO:0043197 ISO
    glutamatergic synapse GO:0098978 IEA
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Arf4 in sEVs
1
Experiment ID 225
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for Arf4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Dao  
Affinity Capture-MS Rattus norvegicus
2 Fbxo21  
Affinity Capture-MS Rattus norvegicus
3 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml



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