Gene description for Myh10
Gene name myosin, heavy chain 10, non-muscle
Gene symbol Myh10
Other names/aliases MCH-B
NMMHC-B
SMemb
Species Rattus norvegicus
 Database cross references - Myh10
ExoCarta ExoCarta_79433
Vesiclepedia VP_79433
Entrez Gene 79433
UniProt Q9JLT0  
 Myh10 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Myh10
Molecular Function
    microfilament motor activity GO:0000146 IBA
    microfilament motor activity GO:0000146 IEA
    microfilament motor activity GO:0000146 ISO
    microfilament motor activity GO:0000146 ISO
    virus receptor activity GO:0001618 IEA
    virus receptor activity GO:0001618 ISO
    cytoskeletal motor activity GO:0003774 IEA
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IEA
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    RNA stem-loop binding GO:0035613 IEA
    RNA stem-loop binding GO:0035613 ISO
    identical protein binding GO:0042802 IDA
    ADP binding GO:0043531 IEA
    ADP binding GO:0043531 ISO
    mRNA 5'-UTR binding GO:0048027 IEA
    mRNA 5'-UTR binding GO:0048027 ISO
    actin filament binding GO:0051015 IBA
    actin filament binding GO:0051015 IEA
    actin filament binding GO:0051015 ISO
    actin filament binding GO:0051015 ISO
Biological Process
    mitotic cytokinesis GO:0000281 IBA
    mitotic cytokinesis GO:0000281 IEA
    mitotic cytokinesis GO:0000281 ISO
    in utero embryonic development GO:0001701 ISO
    neuron migration GO:0001764 ISO
    plasma membrane repair GO:0001778 ISO
    cardiac septum development GO:0003279 ISO
    exocytosis GO:0006887 ISO
    substrate-dependent cell migration, cell extension GO:0006930 ISO
    nuclear migration GO:0007097 ISO
    cell adhesion GO:0007155 IDA
    axonogenesis GO:0007409 ISO
    axon guidance GO:0007411 ISO
    brain development GO:0007420 ISO
    heart development GO:0007507 ISO
    adult heart development GO:0007512 ISO
    regulation of cell shape GO:0008360 IBA
    regulation of cell shape GO:0008360 ISO
    fourth ventricle development GO:0021592 ISO
    lateral ventricle development GO:0021670 ISO
    third ventricle development GO:0021678 ISO
    cerebellar Purkinje cell layer development GO:0021680 ISO
    actin cytoskeleton organization GO:0030036 ISO
    actin filament-based movement GO:0030048 IEA
    actin filament-based movement GO:0030048 ISO
    myofibril assembly GO:0030239 ISO
    actomyosin structure organization GO:0031032 IBA
    actomyosin structure organization GO:0031032 IEA
    actomyosin structure organization GO:0031032 ISO
    neuron projection development GO:0031175 ISO
    aorta development GO:0035904 ISO
    symbiont entry into host cell GO:0046718 IEA
    symbiont entry into host cell GO:0046718 ISO
    positive regulation of protein secretion GO:0050714 IEA
    positive regulation of protein secretion GO:0050714 ISO
    neuromuscular process controlling balance GO:0050885 ISO
    actin filament bundle assembly GO:0051017 IMP
    cardiac myofibril assembly GO:0055003 ISO
    ventricular cardiac muscle cell development GO:0055015 ISO
    cardiac muscle cell proliferation GO:0060038 ISO
    retina development in camera-type eye GO:0060041 ISO
    coronary vasculature development GO:0060976 ISO
    actin filament bundle distribution GO:0070650 IMP
    postsynaptic actin cytoskeleton organization GO:0098974 ISO
    regulation of modification of postsynaptic actin cytoskeleton GO:1905274 IDA
    regulation of modification of postsynaptic actin cytoskeleton GO:1905274 IMP
Subcellular Localization
    stress fiber GO:0001725 IEA
    stress fiber GO:0001725 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    spindle GO:0005819 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    brush border GO:0005903 ISO
    cell cortex GO:0005938 IEA
    cell cortex GO:0005938 ISO
    cytoplasmic side of plasma membrane GO:0009898 ISO
    cell surface GO:0009986 ISO
    myosin complex GO:0016459 IEA
    myosin complex GO:0016459 ISO
    myosin II complex GO:0016460 IBA
    myosin II complex GO:0016460 IEA
    myosin II complex GO:0016460 ISO
    sarcoplasm GO:0016528 ISO
    lamellipodium GO:0030027 IDA
    axon GO:0030424 ISO
    growth cone GO:0030426 ISO
    midbody GO:0030496 IEA
    midbody GO:0030496 ISO
    neuromuscular junction GO:0031594 ISO
    cleavage furrow GO:0032154 IEA
    cleavage furrow GO:0032154 ISO
    myosin filament GO:0032982 IBA
    actomyosin GO:0042641 ISO
    neuron projection GO:0043005 ISO
    neuronal cell body GO:0043025 ISO
    dendritic spine GO:0043197 ISO
    myosin II filament GO:0097513 IEA
    myosin II filament GO:0097513 ISO
    postsynaptic actin cytoskeleton GO:0098871 IDA
    postsynaptic actin cytoskeleton GO:0098871 IEA
    postsynaptic actin cytoskeleton GO:0098871 ISO
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Myh10 in sEVs
1
Experiment ID 90
MISEV standards
EM
Biophysical techniques
HSC70|HSP90|TSG101|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Myh10
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Dao  
Affinity Capture-MS Rattus norvegicus
2 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
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