Gene description for DOK3
Gene name docking protein 3
Gene symbol DOK3
Other names/aliases DOKL
Species Homo sapiens
 Database cross references - DOK3
ExoCarta ExoCarta_79930
Vesiclepedia VP_79930
Entrez Gene 79930
HGNC 24583
MIM 611435
UniProt Q7L591  
 DOK3 identified in sEVs derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for DOK3
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    cell surface receptor protein tyrosine kinase signaling pathway GO:0007169 IBA
    Ras protein signal transduction GO:0007265 IBA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    plasma membrane GO:0005886 TAS
    secretory granule membrane GO:0030667 TAS
    ficolin-1-rich granule membrane GO:0101003 TAS
 Experiment description of studies that identified DOK3 in sEVs
1
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DOK3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PLEKHA3  
Two-hybrid Homo sapiens
2 SHC3  
Two-hybrid Homo sapiens
3 SPAG8  
Two-hybrid Homo sapiens
4 WDR34 89891
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 DPP9 91039
Affinity Capture-MS Homo sapiens
6 COX5B 1329
Two-hybrid Homo sapiens
7 RBPMS 11030
Two-hybrid Homo sapiens
8 GRB2 2885
Affinity Capture-MS Homo sapiens
9 HSPA8 3312
Affinity Capture-MS Homo sapiens
10 YWHAE 7531
Affinity Capture-MS Homo sapiens
11 WDR54  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 DPP8 54878
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 MDFI  
Two-hybrid Homo sapiens
14 HOXA1 3198
Two-hybrid Homo sapiens
15 PPIH 10465
Two-hybrid Homo sapiens
16 LRRK1 79705
Affinity Capture-MS Homo sapiens
17 MIOS 54468
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 UHRF1BP1L 23074
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 A1CF  
Two-hybrid Homo sapiens
20 HSPA2 3306
Affinity Capture-MS Homo sapiens
21 TPP2 7174
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 GCM2  
Two-hybrid Homo sapiens
23 BLNK  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
24 PRKAB2 5565
Two-hybrid Homo sapiens
25 QTRTD1  
Affinity Capture-MS Homo sapiens
26 AMD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 TRAF1 7185
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which DOK3 is involved
PathwayEvidenceSource
Immune System TAS Reactome
Innate Immune System TAS Reactome
Neutrophil degranulation TAS Reactome





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