Gene description for COL21A1
Gene name collagen, type XXI, alpha 1
Gene symbol COL21A1
Other names/aliases COLA1L
FP633
dJ682J15.1
dJ708F5.1
Species Homo sapiens
 Database cross references - COL21A1
ExoCarta ExoCarta_81578
Vesiclepedia VP_81578
Entrez Gene 81578
HGNC 17025
MIM 610002
UniProt Q96P44  
 COL21A1 identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for COL21A1
Molecular Function
    extracellular matrix structural constituent conferring tensile strength GO:0030020 RCA
Subcellular Localization
    extracellular region GO:0005576 TAS
    collagen trimer GO:0005581 IEA
    endoplasmic reticulum lumen GO:0005788 TAS
    cytosol GO:0005829 IDA
    collagen-containing extracellular matrix GO:0062023 HDA
    collagen-containing extracellular matrix GO:0062023 HDA
    collagen-containing extracellular matrix GO:0062023 IBA
 Experiment description of studies that identified COL21A1 in sEVs
1
Experiment ID 237
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 193
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for COL21A1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 COL14A1 7373
Affinity Capture-MS Homo sapiens
2 COLGALT2  
Affinity Capture-MS Homo sapiens
3 PLOD2 5352
Affinity Capture-MS Homo sapiens
4 PLOD3 8985
Affinity Capture-MS Homo sapiens
5 COL4A2 1284
Affinity Capture-MS Homo sapiens
6 PLOD1 5351
Affinity Capture-MS Homo sapiens
7 C1QTNF1 114897
Affinity Capture-MS Homo sapiens
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