Gene description for Csnk2b
Gene name casein kinase 2, beta polypeptide
Gene symbol Csnk2b
Other names/aliases Ck2
Species Rattus norvegicus
 Database cross references - Csnk2b
ExoCarta ExoCarta_81650
Vesiclepedia VP_81650
Entrez Gene 81650
UniProt P67874  
 Csnk2b identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Csnk2b
Molecular Function
    chromatin binding GO:0003682 IEA
    chromatin binding GO:0003682 ISO
    protein serine/threonine kinase activity GO:0004674 IEA
    protein serine/threonine kinase activity GO:0004674 ISO
    signaling receptor binding GO:0005102 IEA
    signaling receptor binding GO:0005102 ISO
    protein kinase regulator activity GO:0019887 IBA
    protein kinase regulator activity GO:0019887 IEA
    protein domain specific binding GO:0019904 IEA
    protein domain specific binding GO:0019904 ISO
    protein-macromolecule adaptor activity GO:0030674 IEA
    protein-macromolecule adaptor activity GO:0030674 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    ribonucleoprotein complex binding GO:0043021 IDA
    metal ion binding GO:0046872 IEA
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 IEA
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 ISO
Biological Process
    Wnt signaling pathway GO:0016055 IEA
    phosphorylation GO:0016310 ISO
    peptidyl-threonine phosphorylation GO:0018107 ISO
    negative regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032435 IEA
    negative regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032435 ISO
    positive regulation of activin receptor signaling pathway GO:0032927 IEA
    positive regulation of activin receptor signaling pathway GO:0032927 ISO
    adiponectin-activated signaling pathway GO:0033211 IEA
    adiponectin-activated signaling pathway GO:0033211 ISO
    response to testosterone GO:0033574 IEP
    negative regulation of blood vessel endothelial cell migration GO:0043537 IEA
    negative regulation of blood vessel endothelial cell migration GO:0043537 ISO
    positive regulation of SMAD protein signal transduction GO:0060391 IEA
    positive regulation of SMAD protein signal transduction GO:0060391 ISO
    endothelial tube morphogenesis GO:0061154 IEA
    endothelial tube morphogenesis GO:0061154 ISO
    symbiont-mediated disruption of host cell PML body GO:0075342 IEA
    symbiont-mediated disruption of host cell PML body GO:0075342 ISO
    liver regeneration GO:0097421 IEP
    negative regulation of viral life cycle GO:1903901 IEA
    negative regulation of viral life cycle GO:1903901 ISO
Subcellular Localization
    chromatin GO:0000785 IDA
    chromatin GO:0000785 IEA
    chromatin GO:0000785 ISO
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    protein kinase CK2 complex GO:0005956 IBA
    protein kinase CK2 complex GO:0005956 IEA
    protein kinase CK2 complex GO:0005956 ISO
    nuclear matrix GO:0016363 IDA
    PML body GO:0016605 IEA
    PML body GO:0016605 ISO
    PcG protein complex GO:0031519 IEA
    PcG protein complex GO:0031519 ISO
    PcG protein complex GO:0031519 ISS
    cell projection GO:0042995 IDA
 Experiment description of studies that identified Csnk2b in sEVs
1
Experiment ID 90
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 95
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 96
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 97
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 98
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Csnk2b
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CALM1  
Biochemical Activity Bos taurus
2 SNCA 6622
Biochemical Activity Homo sapiens
3 Cbx2  
Biochemical Activity Mus musculus
4 Eif4ebp1  
Biochemical Activity Rattus norvegicus
5 Mib1  
Affinity Capture-MS Rattus norvegicus
6 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml
 Pathways in which Csnk2b is involved
PathwayEvidenceSource
Autophagy IEA Reactome
Axon guidance IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Chaperonin-mediated protein folding IEA Reactome
Condensation of Prometaphase Chromosomes IEA Reactome
Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding IEA Reactome
Developmental Biology IEA Reactome
Gene expression (Transcription) IEA Reactome
Generic Transcription Pathway IEA Reactome
Glycerophospholipid biosynthesis IEA Reactome
Immune System IEA Reactome
Innate Immune System IEA Reactome
Intracellular signaling by second messengers IEA Reactome
L1CAM interactions IEA Reactome
M Phase IEA Reactome
Macroautophagy IEA Reactome
Metabolism IEA Reactome
Metabolism of lipids IEA Reactome
Metabolism of proteins IEA Reactome
Mitophagy IEA Reactome
Mitotic Prometaphase IEA Reactome
Nervous system development IEA Reactome
Neutrophil degranulation IEA Reactome
Phospholipid metabolism IEA Reactome
PIP3 activates AKT signaling IEA Reactome
Protein folding IEA Reactome
PTEN Regulation IEA Reactome
Receptor Mediated Mitophagy IEA Reactome
Regulation of PTEN stability and activity IEA Reactome
Regulation of TP53 Activity IEA Reactome
Regulation of TP53 Activity through Phosphorylation IEA Reactome
RNA Polymerase II Transcription IEA Reactome
RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known IEA Reactome
Selective autophagy IEA Reactome
Signal Transduction IEA Reactome
Signal transduction by L1 IEA Reactome
Signaling by WNT IEA Reactome
Synthesis of PC IEA Reactome
TCF dependent signaling in response to WNT IEA Reactome
Transcriptional regulation by RUNX1 IEA Reactome
Transcriptional Regulation by TP53 IEA Reactome
WNT mediated activation of DVL IEA Reactome





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