Gene description for Ndufv2
Gene name NADH dehydrogenase (ubiquinone) flavoprotein 2
Gene symbol Ndufv2
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Ndufv2
ExoCarta ExoCarta_81728
Vesiclepedia VP_81728
Entrez Gene 81728
UniProt P19234  
 Ndufv2 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
 Gene ontology annotations for Ndufv2
Molecular Function
    NADH dehydrogenase activity GO:0003954 IBA
    NADH dehydrogenase (ubiquinone) activity GO:0008137 IEA
    NADH dehydrogenase (ubiquinone) activity GO:0008137 ISO
    NADH dehydrogenase (ubiquinone) activity GO:0008137 ISO
    NADH dehydrogenase (ubiquinone) activity GO:0008137 ISS
    metal ion binding GO:0046872 IEA
    2 iron, 2 sulfur cluster binding GO:0051537 IEA
Biological Process
    mitochondrial electron transport, NADH to ubiquinone GO:0006120 IBA
    mitochondrial electron transport, NADH to ubiquinone GO:0006120 IEA
    mitochondrial electron transport, NADH to ubiquinone GO:0006120 ISO
    mitochondrial electron transport, NADH to ubiquinone GO:0006120 ISS
    nervous system development GO:0007399 ISO
    biological_process GO:0008150 ND
    cardiac muscle tissue development GO:0048738 ISO
    proton transmembrane transport GO:1902600 IEA
Subcellular Localization
    mitochondrion GO:0005739 ISO
    mitochondrion GO:0005739 ISO
    mitochondrial inner membrane GO:0005743 IEA
    mitochondrial inner membrane GO:0005743 ISO
    mitochondrial inner membrane GO:0005743 ISS
    respiratory chain complex I GO:0045271 IBA
    respiratory chain complex I GO:0045271 IEA
    respiratory chain complex I GO:0045271 ISO
    respiratory chain complex I GO:0045271 ISS
 Experiment description of studies that identified Ndufv2 in sEVs
1
Experiment ID 225
MISEV standards
EM
Biophysical techniques
GAPDH|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 94
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Ndufv2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Dao  
Affinity Capture-MS Rattus norvegicus
2 STXBP5L  
Affinity Capture-MS Homo sapiens
3 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml



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