Gene description for Rpl22
Gene name ribosomal protein L22
Gene symbol Rpl22
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Rpl22
ExoCarta ExoCarta_81768
Vesiclepedia VP_81768
Entrez Gene 81768
UniProt P47198  
 Rpl22 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Rpl22
Molecular Function
    RNA binding GO:0003723 IBA
    structural constituent of ribosome GO:0003735 IBA
    structural constituent of ribosome GO:0003735 IEA
    structural constituent of ribosome GO:0003735 ISO
    heparin binding GO:0008201 ISO
    identical protein binding GO:0042802 ISO
Biological Process
    cytoplasmic translation GO:0002181 IBA
    translation GO:0006412 IEA
    biological_process GO:0008150 ND
    alpha-beta T cell differentiation GO:0046632 ISO
    translation at presynapse GO:0140236 ISO
Subcellular Localization
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    ribosome GO:0005840 IDA
    ribosome GO:0005840 IEA
    ribosome GO:0005840 ISO
    cytosolic large ribosomal subunit GO:0022625 IDA
    cytosolic large ribosomal subunit GO:0022625 ISO
    cytosolic ribosome GO:0022626 ISO
    synapse GO:0045202 ISO
    presynapse GO:0098793 ISO
    glutamatergic synapse GO:0098978 ISO
    ribonucleoprotein complex GO:1990904 IEA
    ribonucleoprotein complex GO:1990904 ISO
 Experiment description of studies that identified Rpl22 in sEVs
1
Experiment ID 94
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Rpl22
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Dao  
Affinity Capture-MS Rattus norvegicus
2 Gja1  
Affinity Capture-MS Rattus norvegicus
3 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml



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