Experiment description of studies that identified CAPS in sEVs

1 Experiment ID 34 MISEV standards ✔ EM|IEM Biophysical techniques ✔ TSG101|CD63|MHCI|MHCII Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 19190083     Organism Homo sapiens Experiment description Characterization of exosome-like vesicles released from human tracheobronchial ciliated epithelium: a possible role in innate defense. Authors "Kesimer M, Scull M, Brighton B, Demaria G, Burns K, O'Neal W, Pickles RJ, Sheehan JK" Journal name FASEB Publication year 2009 Sample Tracheobronchial cells Sample name Tracheobronchial epithelial cells Isolation/purification methods Differential centrifugation Filtration Sucrose density gradient Flotation density 1.16 -1.18 g/mL Molecules identified in the study Protein mRNA Methods used in the study Mass spectrometry [QTOF] Western blotting Immunoelectron Microscopy FACS

2 Experiment ID 196 MISEV standards ✔ EM Biophysical techniques ✔ Alix|TSG101|HSP70|CD9 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 22418980     Organism Homo sapiens Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes. Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G." Journal name Kidney Int Publication year 2012 Sample Urine Sample name Urine - Normal high density Isolation/purification methods Differential centrifugation Sucrose cushion Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 197 MISEV standards ✔ EM Biophysical techniques ✔ Alix|TSG101|HSP70|CD9 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 22418980     Organism Homo sapiens Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes. Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G." Journal name Kidney Int Publication year 2012 Sample Urine Sample name Urine - Normal low density Isolation/purification methods Differential centrifugation Sucrose cushion Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for CAPS

Protein Interactor ExoCarta ID Identification method PubMed Species 1 TUBA1A 7846 Affinity Capture-MS Homo sapiens 2 TUBB2A 7280 Affinity Capture-MS Homo sapiens 3 ZC2HC1B   Affinity Capture-MS Homo sapiens 4 TUBA1C 84790 Affinity Capture-MS Homo sapiens 5 TUBA4A 7277 Affinity Capture-MS Homo sapiens 6 TUBAL3 79861 Affinity Capture-MS Homo sapiens 7 TBC1D28   Affinity Capture-MS Homo sapiens 8 TUBB2B 347733 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 9 ZC2HC1A 51101 Affinity Capture-MS Homo sapiens 10 TUBB 203068 Affinity Capture-MS Homo sapiens 11 TUBB3 10381 Affinity Capture-MS Homo sapiens 12 CDH1 999 Proximity Label-MS Homo sapiens 13 TUBB4B 10383 Affinity Capture-MS Homo sapiens 14 TUBB6 84617 Affinity Capture-MS Homo sapiens 15 DLGAP5   Affinity Capture-MS Homo sapiens 16 PRNP 5621 Affinity Capture-MS Homo sapiens 17 IGSF8 93185 Affinity Capture-MS Homo sapiens 18 TUBB8 347688 Affinity Capture-MS Homo sapiens 19 TUBB1 81027 Affinity Capture-MS Homo sapiens 20 TUBB4A 10382 Affinity Capture-MS Homo sapiens View the network

Pathways in which CAPS is involved

No pathways found