Gene description for MIEN1
Gene name migration and invasion enhancer 1
Gene symbol MIEN1
Other names/aliases C17orf37
C35
ORB3
RDX12
XTP4
Species Homo sapiens
 Database cross references - MIEN1
ExoCarta ExoCarta_84299
Vesiclepedia VP_84299
Entrez Gene 84299
HGNC 28230
MIM 611802
UniProt Q9BRT3  
 MIEN1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for MIEN1
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    apoptotic process GO:0006915 IEA
    positive regulation of cell migration GO:0030335 IDA
    negative regulation of apoptotic process GO:0043066 IBA
    negative regulation of apoptotic process GO:0043066 IDA
    positive regulation of filopodium assembly GO:0051491 IBA
    positive regulation of filopodium assembly GO:0051491 IDA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IDA
    cytoplasmic side of plasma membrane GO:0009898 IDA
 Experiment description of studies that identified MIEN1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MIEN1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 VENTX  
Two-hybrid Homo sapiens
2 NXF1 10482
Affinity Capture-RNA Homo sapiens
3 REL 5966
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
4 NLGN3  
Two-hybrid Homo sapiens
5 HLX  
Two-hybrid Homo sapiens
6 PCBP4 57060
Affinity Capture-MS Homo sapiens
7 POU6F2  
Two-hybrid Homo sapiens
8 MOV10 4343
Affinity Capture-RNA Homo sapiens
9 ARID5A  
Two-hybrid Homo sapiens
10 SCCPDH 51097
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 GORASP2 26003
Two-hybrid Homo sapiens
12 DDIT4L  
Two-hybrid Homo sapiens
13 HMGB2 3148
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which MIEN1 is involved
No pathways found





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