Gene description for FBXO18
Gene name F-box protein, helicase, 18
Gene symbol FBXO18
Other names/aliases FBH1
Fbx18
Species Homo sapiens
 Database cross references - FBXO18
ExoCarta ExoCarta_84893
Vesiclepedia VP_84893
Entrez Gene 84893
HGNC 13620
MIM 607222
UniProt Q8NFZ0  
 FBXO18 identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for FBXO18
Molecular Function
    DNA helicase activity GO:0003678 IDA
    DNA helicase activity GO:0003678 IMP
    DNA clamp loader activity GO:0003689 IEA
    double-stranded DNA binding GO:0003690 IDA
    single-stranded DNA binding GO:0003697 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    four-way junction helicase activity GO:0009378 IEA
    DNA translocase activity GO:0015616 IDA
    isomerase activity GO:0016853 IEA
    ATP hydrolysis activity GO:0016887 IEA
    double-stranded DNA helicase activity GO:0036121 IEA
    3'-5' DNA helicase activity GO:0043138 IBA
    3'-5' DNA helicase activity GO:0043138 IDA
    forked DNA-dependent helicase activity GO:0061749 IEA
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
    single-stranded 3'-5' DNA helicase activity GO:1990518 IEA
Biological Process
    double-strand break repair via homologous recombination GO:0000724 IBA
    double-strand break repair via homologous recombination GO:0000724 ISS
    positive regulation of protein phosphorylation GO:0001934 IMP
    DNA repair GO:0006281 IDA
    DNA catabolic process GO:0006308 IMP
    chromatin remodeling GO:0006338 IEA
    DNA damage response GO:0006974 IDA
    protein ubiquitination GO:0016567 IDA
    protein ubiquitination GO:0016567 IEA
    replication fork processing GO:0031297 IBA
    replication fork processing GO:0031297 IDA
    DNA duplex unwinding GO:0032508 IEA
    response to intra-S DNA damage checkpoint signaling GO:0072429 IMP
    chromatin looping GO:0140588 IEA
    positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage GO:1902231 IMP
    negative regulation of double-strand break repair via homologous recombination GO:2000042 IDA
Subcellular Localization
    chromatin GO:0000785 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    SCF ubiquitin ligase complex GO:0019005 IDA
 Experiment description of studies that identified FBXO18 in sEVs
1
Experiment ID 234
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for FBXO18
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CUL1 8454
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
2 DTL  
Affinity Capture-Western Homo sapiens
3 MRRF  
Affinity Capture-MS Homo sapiens
4 ESR1  
Reconstituted Complex Homo sapiens
5 ILKAP 80895
Affinity Capture-MS Homo sapiens
6 RPA2 6118
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
7 ZDHHC17 23390
Two-hybrid Homo sapiens
8 RPA1 6117
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
9 SKP1 6500
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
10 UBC 7316
Biochemical Activity Homo sapiens
11 RBX1 9978
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
12 Rock1  
Affinity Capture-MS Mus musculus
13 RALA 5898
Affinity Capture-MS Homo sapiens
14 PCNA 5111
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
15 APEX1 328
Affinity Capture-RNA Homo sapiens
16 PRR3  
Affinity Capture-MS Homo sapiens
17 MYC  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
18 RPA3 6119
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
19 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
20 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
21 PTGES3 10728
Affinity Capture-MS Homo sapiens
22 TTC9C 283237
Affinity Capture-MS Homo sapiens
23 Nedd1  
Affinity Capture-MS Mus musculus
24 RAD51  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
25 CCND3  
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which FBXO18 is involved
No pathways found





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