ExoCarta: Gene summary

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Gene description for LGR5
Gene name	leucine-rich repeat containing G protein-coupled receptor 5
Gene symbol	LGR5
Other names/aliases	FEX GPR49 GPR67 GRP49 HG38
Species	Homo sapiens
Database cross references - LGR5
ExoCarta	ExoCarta_8549
Vesiclepedia	VP_8549
Entrez Gene	8549
HGNC	4504
MIM	606667
UniProt	O75473
LGR5 identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells	34887515

Gene ontology annotations for LGR5

Molecular Function
	transmembrane signaling receptor activity	GO:0004888	IDA
	G protein-coupled receptor activity	GO:0004930	IDA
	protein binding	GO:0005515	IPI
	protein-hormone receptor activity	GO:0016500	IEA
Biological Process
	hair follicle development	GO:0001942	IEA
	G protein-coupled receptor signaling pathway	GO:0007186	TAS
	oocyte differentiation	GO:0009994	IEA
	regulation of cell population proliferation	GO:0042127	IEA
	inner ear development	GO:0048839	IEA
	positive regulation of canonical Wnt signaling pathway	GO:0090263	IBA
	positive regulation of canonical Wnt signaling pathway	GO:0090263	IDA
	epithelial cell proliferation involved in renal tubule morphogenesis	GO:2001013	IEA
Subcellular Localization
	nucleoplasm	GO:0005654	IDA
	Golgi apparatus	GO:0005794	IDA
	plasma membrane	GO:0005886	IBA
	plasma membrane	GO:0005886	IDA
	plasma membrane	GO:0005886	TAS
	trans-Golgi network membrane	GO:0032588	IDA

Experiment description of studies that identified LGR5 in sEVs

Experiment ID

1203

MISEV standards
✔ EM	Biophysical techniques
✔ SDCBP\|FLOT1\|CD9\|CD81\|CD63\|EPCAM\|GAPDH\|LAMP1\|TFRC\|CD151\|CD82\|LAMP2\|RAB35\|TSG101\|FLOT2\|RAB5B\|ICAM1\|RAB5A	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

34887515

Organism

Homo sapiens

Experiment description

Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets

Authors

"Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."

Journal name

Nat Cell Biol

Publication year

2021

Sample

Colorectal cancer cells

Sample name

DiFi

Isolation/purification methods

Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation

Flotation density

Molecules identified in the study

Protein
miRNA

Methods used in the study

Western blotting
Mass spectrometry
RNA sequencing

Protein-protein interactions for LGR5

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

Rspo2

Co-crystal Structure

Mus musculus

Znrf3

Reconstituted Complex

Mus musculus

ZNRF3

Co-crystal Structure

Homo sapiens

CNPY3

10695

Affinity Capture-MS

Homo sapiens

NTRK1

4914

Affinity Capture-MS

Homo sapiens

LEMD2

221496

Affinity Capture-MS

Homo sapiens

DHPS

1725

Affinity Capture-MS

Homo sapiens

NBR1

4077

Affinity Capture-MS

Homo sapiens

View the network

Pathways in which LGR5 is involved

Pathway	Evidence	Source
Regulation of FZD by ubiquitination	TAS	Reactome
Signal Transduction	TAS	Reactome
Signaling by WNT	TAS	Reactome
TCF dependent signaling in response to WNT	TAS	Reactome

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