Experiment description of studies that identified BTAF1 in sEVs

1 Experiment ID 1333 MISEV standards Biophysical techniques ✔ CD81|EPCAM|CD9 Enriched markers ✘ Negative markers Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35918900     Organism Homo sapiens Experiment description Characterization of protein complexes in extracellular vesicles by intact extracellular vesicle crosslinking mass spectrometry (iEVXL) Authors "Bauzá-Martinez J, Armony G, Pronker MF, Wu W. " Journal name J Extracell Vesicles Publication year 2022 Sample Breast cancer cells Sample name MDA-MB-231 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectometry Western blotting

2 Experiment ID 1335 MISEV standards Biophysical techniques ✔ EPCAM|CD9 Enriched markers ✘ Negative markers Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35918900     Organism Homo sapiens Experiment description Characterization of protein complexes in extracellular vesicles by intact extracellular vesicle crosslinking mass spectrometry (iEVXL) Authors "Bauzá-Martinez J, Armony G, Pronker MF, Wu W. " Journal name J Extracell Vesicles Publication year 2022 Sample Breast cancer cells Sample name MDA-MB-231 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectometry

3 Experiment ID 488 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36408942     Organism Homo sapiens Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." Journal name Proteomics Publication year 2023 Sample Mesenchymal stem cells Sample name UCMSC Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

4 Experiment ID 217 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|CD81|CD9|CD63 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23844026     Organism Homo sapiens Experiment description Characterization of human thymic exosomes. Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O." Journal name PLoS One Publication year 2013 Sample Thymus Sample name Normal-Thymus Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for BTAF1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 C5AR2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 2 LYPD3 27076 Affinity Capture-MS Homo sapiens 3 PIH1D3   Two-hybrid Homo sapiens 4 TRRAP 8295 Co-fractionation Homo sapiens 5 HCST   Affinity Capture-MS Homo sapiens 6 SLC2A1 6513 Affinity Capture-MS Homo sapiens 7 SPSB4   Affinity Capture-MS Homo sapiens 8 FPR1   Affinity Capture-MS Homo sapiens 9 EDNRB 1910 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 10 C5AR1   Affinity Capture-MS Homo sapiens 11 FFAR1   Affinity Capture-MS Homo sapiens 12 PNKD   Affinity Capture-MS Homo sapiens 13 SLC18A2   Affinity Capture-MS Homo sapiens 14 VIPR2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 15 HLA-B 3106 Affinity Capture-MS Homo sapiens 16 CLEC2B   Affinity Capture-MS Homo sapiens 17 SEMA4C 54910 Affinity Capture-MS Homo sapiens 18 EPHA1 2041 Affinity Capture-MS Homo sapiens 19 GRPR   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 20 F2RL1   Affinity Capture-MS Homo sapiens 21 PTH1R   Affinity Capture-MS Homo sapiens 22 GPR17 2840 Affinity Capture-MS Homo sapiens 23 BSG 682 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 24 GPR12   Affinity Capture-MS Homo sapiens 25 CD70 970 Affinity Capture-MS Homo sapiens 26 GCGR   Affinity Capture-MS Homo sapiens 27 CUL3 8452 Affinity Capture-MS Homo sapiens 28 CTDP1 9150 Affinity Capture-MS Homo sapiens 29 FOXI2   Affinity Capture-MS Homo sapiens 30 PIAS4   Two-hybrid Homo sapiens 31 P2RY8   Affinity Capture-MS Homo sapiens 32 CBX3 11335 Proximity Label-MS Homo sapiens 33 COMTD1 118881 Affinity Capture-MS Homo sapiens 34 FOXL2   Affinity Capture-MS Homo sapiens 35 NTRK1 4914 Affinity Capture-MS Homo sapiens 36 GPR182   Affinity Capture-MS Homo sapiens 37 TEAD2   Affinity Capture-MS Homo sapiens 38 RHOT2 89941 Affinity Capture-MS Homo sapiens 39 MLNR   Affinity Capture-MS Homo sapiens 40 FOXH1   Affinity Capture-MS Homo sapiens 41 S1PR1 1901 Affinity Capture-MS Homo sapiens 42 SLC15A1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 43 CYB5B 80777 Affinity Capture-MS Homo sapiens 44 EFNA4   Affinity Capture-MS Homo sapiens 45 APLNR   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 46 CXCR4 7852 Affinity Capture-MS Homo sapiens 47 FOXG1   Affinity Capture-MS Homo sapiens 48 SIGLECL1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 49 BTNL9   Affinity Capture-MS Homo sapiens 50 CD83   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 51 FZD10   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 52 NPTN 27020 Affinity Capture-MS Homo sapiens 53 PTH2R   Affinity Capture-MS Homo sapiens 54 CMKLR1 1240 Affinity Capture-MS Homo sapiens 55 MGARP   Affinity Capture-MS Homo sapiens 56 TFAP4 7023 Affinity Capture-MS Homo sapiens 57 NPY2R   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 58 EGFR 1956 Affinity Capture-MS Homo sapiens 59 HTR2C   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 60 FOXL1   Affinity Capture-MS Homo sapiens 61 AQP3   Affinity Capture-MS Homo sapiens 62 OPRL1 4987 Affinity Capture-MS Homo sapiens 63 ADAM7   Affinity Capture-MS Homo sapiens 64 TBP   Reconstituted Complex Homo sapiens Affinity Capture-MS Homo sapiens Reconstituted Complex Homo sapiens Affinity Capture-Western Homo sapiens Co-fractionation Homo sapiens 65 C3orf18   Affinity Capture-MS Homo sapiens 66 RAB2B 84932 Affinity Capture-MS Homo sapiens 67 GPR45   Affinity Capture-MS Homo sapiens 68 GTF2H4 2968 Co-purification Homo sapiens 69 FOXQ1   Affinity Capture-MS Homo sapiens 70 VIPR1   Affinity Capture-MS Homo sapiens 71 TNFSF13B   Affinity Capture-MS Homo sapiens 72 FAXC   Affinity Capture-MS Homo sapiens 73 NFATC2   Affinity Capture-MS Homo sapiens 74 CCDC8   Affinity Capture-MS Homo sapiens 75 AVPR2   Affinity Capture-MS Homo sapiens 76 HIF1A 3091 Affinity Capture-MS Homo sapiens 77 MAS1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 78 PLEKHA4 57664 Affinity Capture-MS Homo sapiens 79 OCIAD1 54940 Affinity Capture-MS Homo sapiens 80 PARP1 142 Proximity Label-MS Homo sapiens 81 BKRF1   Affinity Capture-MS 82 MVD 4597 Two-hybrid Homo sapiens Affinity Capture-Western Homo sapiens 83 OPALIN   Affinity Capture-MS Homo sapiens 84 MFSD4   Affinity Capture-MS Homo sapiens 85 FOXB1   Affinity Capture-MS Homo sapiens 86 MEN1 4221 Affinity Capture-MS Homo sapiens 87 LRRN4CL 221091 Affinity Capture-MS Homo sapiens 88 ELAVL1 1994 Affinity Capture-RNA Homo sapiens 89 MAGEA9   Affinity Capture-MS Homo sapiens 90 CD80 941 Affinity Capture-MS Homo sapiens 91 NUP50 10762 Proximity Label-MS Homo sapiens 92 SNW1 22938 Affinity Capture-MS Homo sapiens 93 LPAR4   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 94 C9orf72   Affinity Capture-MS Homo sapiens 95 FOXE1   Affinity Capture-MS Homo sapiens 96 RPA3 6119 Proximity Label-MS Homo sapiens 97 EP300 2033 Affinity Capture-MS Homo sapiens 98 CDC5L 988 Affinity Capture-MS Homo sapiens 99 HLA-C 3107 Affinity Capture-MS Homo sapiens View the network

Pathways in which BTAF1 is involved

No pathways found