Experiment description of studies that identified TMSB10 in sEVs

1 Experiment ID 226 MISEV standards ✔ EM Biophysical techniques ✔ GAPDH|CD9|FLOT1 Enriched markers ✔ LMNA|H2AFX|ATP5A1|TOMM20 Negative markers ✘ Particle analysis Identified molecule mRNA Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa) PubMed ID 26027894     Organism Homo sapiens Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting" Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar" Journal name Journal of Extracellular Vesicles Publication year 2015 Sample Endothelial cells Sample name HMEC-1 Isolation/purification methods Differential ultracentrifugation Sucrose density gradient Flotation density 1.10 g/mL Molecules identified in the study miRNA Protein snoRNA lncRNA yRNA snRNA mRNA ncRNA mtRNA vtRNA scaRNA lincRNA Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa) Western blotting

2 Experiment ID 234 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name HKCI-C3 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

3 Experiment ID 235 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name HKCI-8 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

4 Experiment ID 236 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name MHCC97L Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

5 Experiment ID 237 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocytes Sample name MIHA Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RNA Sequencing

Protein-protein interactions for TMSB10

Protein Interactor ExoCarta ID Identification method PubMed Species 1 GSTP1 2950 Affinity Capture-MS Homo sapiens 2 BAG3 9531 Affinity Capture-Luminescence Homo sapiens 3 COPG2 26958 Cross-Linking-MS (XL-MS) Homo sapiens 4 PKN2 5586 Cross-Linking-MS (XL-MS) Homo sapiens 5 TERF2   Two-hybrid Homo sapiens 6 LRRC20   Cross-Linking-MS (XL-MS) Homo sapiens 7 POT1   Two-hybrid Homo sapiens 8 TMSB4Y   Cross-Linking-MS (XL-MS) Homo sapiens Cross-Linking-MS (XL-MS) Homo sapiens Cross-Linking-MS (XL-MS) Homo sapiens 9 INPPL1 3636 Affinity Capture-MS Homo sapiens 10 PFN1 5216 Co-fractionation Homo sapiens 11 RPL35 11224 Cross-Linking-MS (XL-MS) Homo sapiens 12 ALAS1   Two-hybrid Homo sapiens Two-hybrid Homo sapiens 13 AHNAK 79026 Cross-Linking-MS (XL-MS) Homo sapiens 14 LGALS3 3958 Co-fractionation Homo sapiens 15 DNAJB1 3337 Affinity Capture-MS Homo sapiens 16 VPS45 11311 Affinity Capture-MS Homo sapiens 17 TERF2IP 54386 Two-hybrid Homo sapiens 18 TERF1 7013 Two-hybrid Homo sapiens 19 ACSL3 2181 Cross-Linking-MS (XL-MS) Homo sapiens 20 DCUN1D1 54165 Cross-Linking-MS (XL-MS) Homo sapiens 21 NR2E1   Affinity Capture-MS Homo sapiens 22 KRTAP9-2   Two-hybrid Homo sapiens 23 ACTB 60 Affinity Capture-MS Homo sapiens 24 TMSB4X 7114 Cross-Linking-MS (XL-MS) Homo sapiens 25 PIK3C2A 5286 Affinity Capture-MS Homo sapiens 26 LMNA 4000 Affinity Capture-MS Homo sapiens 27 ACSL4 2182 Cross-Linking-MS (XL-MS) Homo sapiens 28 EFEMP1 2202 Affinity Capture-MS Homo sapiens 29 YRDC 79693 Co-fractionation Homo sapiens 30 SLC25A5 292 Cross-Linking-MS (XL-MS) Homo sapiens View the network

Pathways in which TMSB10 is involved

No pathways found