Gene description for CHRDL1
Gene name chordin-like 1
Gene symbol CHRDL1
Other names/aliases CHL
MGC1
MGCN
NRLN1
VOPT
dA141H5.1
Species Homo sapiens
 Database cross references - CHRDL1
ExoCarta ExoCarta_91851
Vesiclepedia VP_91851
Entrez Gene 91851
HGNC 29861
MIM 300350
UniProt Q9BU40  
 CHRDL1 identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for CHRDL1
Molecular Function
    BMP binding GO:0036122 IBA
    transforming growth factor beta binding GO:0050431 IEA
Biological Process
    embryonic axis specification GO:0000578 IEA
    ossification GO:0001503 IEA
    eye development GO:0001654 IMP
    cell differentiation GO:0030154 IBA
    BMP signaling pathway GO:0030509 IEA
    negative regulation of BMP signaling pathway GO:0030514 IBA
    regulation of synaptic plasticity GO:0048167 IEA
    synapse maturation GO:0060074 IEA
    AMPA glutamate receptor clustering GO:0097113 IEA
    excitatory chemical synaptic transmission GO:0098976 IEA
Subcellular Localization
    extracellular region GO:0005576 TAS
    endoplasmic reticulum lumen GO:0005788 TAS
    plasma membrane GO:0005886 IEA
    synapse GO:0045202 IEA
 Experiment description of studies that identified CHRDL1 in sEVs
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for CHRDL1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HECTD3 79654
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 SNX27 81609
Affinity Capture-MS Homo sapiens
3 ZZEF1 23140
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 CALCOCO2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 UBR1 197131
Affinity Capture-MS Homo sapiens
6 LRP1B 53353
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 CREBBP  
Two-hybrid Homo sapiens
8 CBWD3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here