Gene description for DHRS3
Gene name dehydrogenase/reductase (SDR family) member 3
Gene symbol DHRS3
Other names/aliases DD83.1
RDH17
Rsdr1
SDR1
SDR16C1
retSDR1
Species Homo sapiens
 Database cross references - DHRS3
ExoCarta ExoCarta_9249
Vesiclepedia VP_9249
Entrez Gene 9249
HGNC 17693
MIM 612830
UniProt O75911  
 DHRS3 identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for DHRS3
Molecular Function
    nucleotide binding GO:0000166 TAS
    all-trans-retinol dehydrogenase (NAD+) activity GO:0004745 IBA
    electron transfer activity GO:0009055 TAS
    all-trans-retinol dehydrogenase (NADP+) activity GO:0052650 TAS
Biological Process
    retinoid metabolic process GO:0001523 IBA
    retinoid metabolic process GO:0001523 TAS
    outflow tract morphogenesis GO:0003151 IEA
    visual perception GO:0007601 TAS
    regulation of ossification GO:0030278 IEA
    retinol metabolic process GO:0042572 TAS
    regulation of retinoic acid receptor signaling pathway GO:0048385 IBA
    negative regulation of retinoic acid receptor signaling pathway GO:0048387 IEA
    roof of mouth development GO:0060021 IEA
    bone morphogenesis GO:0060349 IEA
    cardiac septum morphogenesis GO:0060411 IEA
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 TAS
    lipid droplet GO:0005811 IBA
    photoreceptor outer segment membrane GO:0042622 TAS
 Experiment description of studies that identified DHRS3 in sEVs
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for DHRS3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NKAIN1  
Affinity Capture-MS Homo sapiens
2 C5AR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 CYSTM1 84418
Affinity Capture-MS Homo sapiens
4 PLS1 5357
Affinity Capture-MS Homo sapiens
5 CLPX 10845
Affinity Capture-MS Homo sapiens
6 FEM1A  
Affinity Capture-MS Homo sapiens
7 OR10H2  
Affinity Capture-MS Homo sapiens
8 SIDT2 51092
Affinity Capture-MS Homo sapiens
9 CCNYL1 151195
Affinity Capture-MS Homo sapiens
10 ZDHHC12  
Affinity Capture-MS Homo sapiens
11 PTCH2  
Affinity Capture-MS Homo sapiens
12 IL22RA1 58985
Affinity Capture-MS Homo sapiens
13 ARL8A 127829
Affinity Capture-MS Homo sapiens
14 HRAS 3265
Affinity Capture-MS Homo sapiens
15 TLR5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
17 GAP43  
Affinity Capture-MS Homo sapiens
18 IL4R  
Affinity Capture-MS Homo sapiens
19 PCDHGB2  
Affinity Capture-MS Homo sapiens
20 MED21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 GNG4  
Affinity Capture-MS Homo sapiens
22 CD59 966
Affinity Capture-MS Homo sapiens
23 COPB2 9276
Affinity Capture-MS Homo sapiens
24 DHRS4 10901
Affinity Capture-MS Homo sapiens
25 POLDIP2 26073
Affinity Capture-MS Homo sapiens
26 TGFA  
Affinity Capture-MS Homo sapiens
27 MARCH4  
Affinity Capture-MS Homo sapiens
28 SRC 6714
Affinity Capture-MS Homo sapiens
29 IL17RC 84818
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 SLC22A4 6583
Affinity Capture-MS Homo sapiens
31 PIGH  
Affinity Capture-MS Homo sapiens
32 CCNY 219771
Affinity Capture-MS Homo sapiens
33 C3orf52  
Affinity Capture-MS Homo sapiens
34 C12orf75  
Affinity Capture-MS Homo sapiens
35 HINT3 135114
Affinity Capture-MS Homo sapiens
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