Gene description for GTF3C5
Gene name general transcription factor IIIC, polypeptide 5, 63kDa
Gene symbol GTF3C5
Other names/aliases TFIIIC63
TFIIICepsilon
TFiiiC2-63
Species Homo sapiens
 Database cross references - GTF3C5
ExoCarta ExoCarta_9328
Vesiclepedia VP_9328
Entrez Gene 9328
HGNC 4668
MIM 604890
UniProt Q9Y5Q8  
 GTF3C5 identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Thymus 23844026    
 Gene ontology annotations for GTF3C5
Molecular Function
    RNA polymerase III general transcription initiation factor activity GO:0000995 IDA
    RNA polymerase III type 1 promoter sequence-specific DNA binding GO:0001002 IBA
    RNA polymerase III type 2 promoter sequence-specific DNA binding GO:0001003 IBA
    DNA binding GO:0003677 IC
    protein binding GO:0005515 IPI
Biological Process
    transcription by RNA polymerase III GO:0006383 IC
    transcription initiation at RNA polymerase III promoter GO:0006384 IEA
    skeletal muscle cell differentiation GO:0035914 IEA
    5S class rRNA transcription by RNA polymerase III GO:0042791 IC
    tRNA transcription by RNA polymerase III GO:0042797 IC
Subcellular Localization
    transcription factor TFIIIC complex GO:0000127 IBA
    transcription factor TFIIIC complex GO:0000127 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified GTF3C5 in sEVs
1
Experiment ID 207
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
MISEV standards
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GTF3C5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 HIST2H2BE 8349
Affinity Capture-MS Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 KRTAP12-3  
Two-hybrid Homo sapiens
5 SPRTN  
Affinity Capture-MS Homo sapiens
6 COIL  
Proximity Label-MS Homo sapiens
7 PAXIP1  
Affinity Capture-MS Homo sapiens
8 CIB2  
Affinity Capture-MS Homo sapiens
9 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
10 MDFI  
Two-hybrid Homo sapiens
11 C9orf78 51759
Affinity Capture-MS Homo sapiens
12 CSNK2A1 1457
Affinity Capture-MS Homo sapiens
13 LOXL4 84171
Affinity Capture-MS Homo sapiens
14 SOX2  
Affinity Capture-MS Homo sapiens
15 CENPA  
Proximity Label-MS Homo sapiens
16 Ncapg2  
Affinity Capture-MS Mus musculus
17 USP36  
Affinity Capture-MS Homo sapiens
18 ZNF44  
Affinity Capture-MS Homo sapiens
19 SRA1 10011
Far Western Homo sapiens
20 RNPS1 10921
Affinity Capture-MS Homo sapiens
21 FBXW7  
Affinity Capture-MS Homo sapiens
22 KRT40  
Two-hybrid Homo sapiens
23 FBL 2091
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 Rfx5  
Affinity Capture-MS Mus musculus
25 MAD2L2 10459
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 SLX4  
Affinity Capture-MS Homo sapiens
27 MECP2 4204
Affinity Capture-MS Homo sapiens
28 GTF3A 2971
Affinity Capture-MS Homo sapiens
29 CUL1 8454
Affinity Capture-MS Homo sapiens
30 CSNK2B 1460
Affinity Capture-MS Homo sapiens
31 FBXW5  
Affinity Capture-MS Homo sapiens
32 RBPJ 3516
Affinity Capture-MS Homo sapiens
33 HSPBP1 23640
Affinity Capture-MS Homo sapiens
34 Gtf3c4  
Affinity Capture-MS Mus musculus
35 Gtf3c2  
Affinity Capture-MS Mus musculus
36 PLBD1 79887
Affinity Capture-MS Homo sapiens
37 TAF10 6881
Affinity Capture-MS Homo sapiens
38 PARK2  
Affinity Capture-MS Homo sapiens
39 KLHL34  
Affinity Capture-MS Homo sapiens
40 PRPS2 5634
Affinity Capture-MS Homo sapiens
41 VCP 7415
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 NTRK1 4914
Affinity Capture-MS Homo sapiens
43 IFI16 3428
Affinity Capture-MS Homo sapiens
44 GTF3C2  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
45 PNMA2  
Affinity Capture-MS Homo sapiens
46 RPA4  
Proximity Label-MS Homo sapiens
47 HECTD1 25831
Affinity Capture-MS Homo sapiens
48 SPANXN2  
Affinity Capture-MS Homo sapiens
49 ETS2  
Affinity Capture-MS Homo sapiens
50 NIPSNAP1 8508
Affinity Capture-MS Homo sapiens
51 FOXS1  
Affinity Capture-MS Homo sapiens
52 UBXN6 80700
Affinity Capture-MS Homo sapiens
53 UPP1 7378
Affinity Capture-MS Homo sapiens
54 CD3EAP  
Proximity Label-MS Homo sapiens
55 NOTCH2NL 388677
Two-hybrid Homo sapiens
56 BRD7  
Co-fractionation Homo sapiens
57 OGT 8473
Reconstituted Complex Homo sapiens
58 BRF1  
Affinity Capture-Western Homo sapiens
59 GTF3C3 9330
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 TUBB 203068
Affinity Capture-MS Homo sapiens
61 PPM1M  
Affinity Capture-MS Homo sapiens
62 FOXG1  
Affinity Capture-MS Homo sapiens
63 SMARCAD1  
Affinity Capture-MS Homo sapiens
64 XPO1 7514
Affinity Capture-MS Homo sapiens
65 ECT2 1894
Affinity Capture-MS Homo sapiens
66 GTF3C1  
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
67 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 NR3C1 2908
Proximity Label-MS Homo sapiens
69 PHKG2 5261
Affinity Capture-MS Homo sapiens
70 FOXL1  
Affinity Capture-MS Homo sapiens
71 KRTAP10-3  
Two-hybrid Homo sapiens
72 PLK1 5347
Affinity Capture-MS Homo sapiens
73 SNX27 81609
Affinity Capture-MS Homo sapiens
74 GTF3C4 9329
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
75 TBP  
Reconstituted Complex Homo sapiens
76 CEP250 11190
Affinity Capture-MS Homo sapiens
77 GTF3C6  
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
78 RPA3 6119
Proximity Label-MS Homo sapiens
79 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
80 TOP1 7150
Co-fractionation Homo sapiens
81 DDRGK1 65992
Affinity Capture-MS Homo sapiens
82 SF3B3 23450
Affinity Capture-MS Homo sapiens
83 FOXQ1  
Affinity Capture-MS Homo sapiens
84 Rpl35 66489
Affinity Capture-MS Mus musculus
85 NFATC2  
Affinity Capture-MS Homo sapiens
86 C9orf72  
Affinity Capture-MS Homo sapiens
87 CCDC8  
Affinity Capture-MS Homo sapiens
88 RPS16 6217
Affinity Capture-MS Homo sapiens
89 Gtf3c6  
Affinity Capture-MS Mus musculus
90 Cbx1  
Affinity Capture-MS Mus musculus
91 DNMT3L  
Reconstituted Complex Homo sapiens
92 TCF21  
Two-hybrid Homo sapiens
93 PARP1 142
Proximity Label-MS Homo sapiens
94 FBXL6  
Affinity Capture-MS Homo sapiens
95 POLR3C 10623
Affinity Capture-Western Homo sapiens
96 HDAC6 10013
Affinity Capture-MS Homo sapiens
97 Rbm8a  
Affinity Capture-MS Mus musculus
98 Gtf3c3  
Affinity Capture-MS Mus musculus
99 PBRM1 55193
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
100 UFL1 23376
Affinity Capture-MS Homo sapiens
101 TCF4  
Two-hybrid Homo sapiens
102 FOXB1  
Affinity Capture-MS Homo sapiens
103 METTL3  
Affinity Capture-MS Homo sapiens
104 SERBP1 26135
Affinity Capture-MS Homo sapiens
105 PTGES3 10728
Affinity Capture-MS Homo sapiens
106 KRAS 3845
Negative Genetic Homo sapiens
107 CD44 960
Affinity Capture-MS Homo sapiens
108 KDM5C  
Affinity Capture-MS Homo sapiens
109 EP300 2033
Affinity Capture-Western Homo sapiens
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