Gene description for SLC22A8
Gene name solute carrier family 22 (organic anion transporter), member 8
Gene symbol SLC22A8
Other names/aliases OAT3
Species Homo sapiens
 Database cross references - SLC22A8
ExoCarta ExoCarta_9376
Vesiclepedia VP_9376
Entrez Gene 9376
HGNC 10972
MIM 607581
UniProt Q8TCC7  
 SLC22A8 identified in sEVs derived from the following tissue/cell type
Prostate cancer cells 25844599    
Urine 19056867    
 Gene ontology annotations for SLC22A8
Molecular Function
    solute:inorganic anion antiporter activity GO:0005452 IDA
    organic anion transmembrane transporter activity GO:0008514 IDA
    organic anion transmembrane transporter activity GO:0008514 TAS
    prostaglandin transmembrane transporter activity GO:0015132 IDA
    xenobiotic transmembrane transporter activity GO:0042910 IDA
Biological Process
    monoatomic ion transport GO:0006811 IEA
    response to toxic substance GO:0009636 IEA
    inorganic anion transport GO:0015698 IEA
    organic anion transport GO:0015711 IBA
    prostaglandin transport GO:0015732 IDA
    xenobiotic transport GO:0042908 IEA
    transmembrane transport GO:0055085 IEA
    transport across blood-brain barrier GO:0150104 NAS
Subcellular Localization
    plasma membrane GO:0005886 TAS
    basolateral plasma membrane GO:0016323 IDA
    basolateral plasma membrane GO:0016323 ISS
    apical plasma membrane GO:0016324 IDA
    apical plasma membrane GO:0016324 ISS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified SLC22A8 in sEVs
1
Experiment ID 274
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
2
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for SLC22A8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NDFIP1 80762
Affinity Capture-MS Homo sapiens
2 GK  
Affinity Capture-MS Homo sapiens
3 TVP23C  
Affinity Capture-MS Homo sapiens
4 ORMDL1 94101
Affinity Capture-MS Homo sapiens
5 TMUB2  
Affinity Capture-MS Homo sapiens
6 MTX3  
Affinity Capture-MS Homo sapiens
7 NOMO1 23420
Affinity Capture-MS Homo sapiens
8 ABHD17B 51104
Affinity Capture-MS Homo sapiens
9 NEDD4 4734
Affinity Capture-Western Homo sapiens
10 SAMM50 25813
Affinity Capture-MS Homo sapiens
11 MTFP1  
Affinity Capture-MS Homo sapiens
12 MYC  
Affinity Capture-MS Homo sapiens
13 FAM91A1 157769
Affinity Capture-MS Homo sapiens
14 EPHA5 2044
Affinity Capture-MS Homo sapiens
15 CD320 51293
Affinity Capture-MS Homo sapiens
16 ATL3 25923
Affinity Capture-MS Homo sapiens
17 NEDD4L 23327
Affinity Capture-Western Homo sapiens
18 HLA-C 3107
Affinity Capture-MS Homo sapiens
19 SLC25A46 91137
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here