Gene description for Prdx6
Gene name peroxiredoxin 6
Gene symbol Prdx6
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Prdx6
ExoCarta ExoCarta_94167
Vesiclepedia VP_94167
Entrez Gene 94167
UniProt O35244  
 Prdx6 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Urine 20535238    
 Gene ontology annotations for Prdx6
Molecular Function
    peroxidase activity GO:0004601 IBA
    peroxidase activity GO:0004601 ISO
    glutathione peroxidase activity GO:0004602 IDA
    glutathione peroxidase activity GO:0004602 ISO
    phospholipase A2 activity GO:0004623 ISO
    protein binding GO:0005515 IPI
    ubiquitin protein ligase binding GO:0031625 ISO
    identical protein binding GO:0042802 ISO
    1-acylglycerophosphocholine O-acyltransferase activity GO:0047184 IDA
    1-acylglycerophosphocholine O-acyltransferase activity GO:0047184 ISO
    calcium-dependent phospholipase A2 activity GO:0047498 IEA
    calcium-independent phospholipase A2 activity GO:0047499 ISO
    peroxiredoxin activity GO:0051920 IEA
Biological Process
    response to reactive oxygen species GO:0000302 ISO
    response to oxidative stress GO:0006979 ISO
    bleb assembly GO:0032060 ISO
    hydrogen peroxide catabolic process GO:0042744 IDA
    cell redox homeostasis GO:0045454 IBA
    glycerophospholipid catabolic process GO:0046475 ISO
    positive regulation of mRNA splicing, via spliceosome GO:0048026 ISO
    cellular oxidant detoxification GO:0098869 IEA
    cellular oxidant detoxification GO:0098869 ISO
Subcellular Localization
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    lysosome GO:0005764 IEA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
 Experiment description of studies that identified Prdx6 in sEVs
1
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 192
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|HSP70|CD63|CD81|FLOT
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M."
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Prdx6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MAPK1 5594
Biochemical Activity Homo sapiens
2 Sftpa1  
Affinity Capture-Western Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
3 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
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