|
 Gene description for Nans |
| Gene name |
N-acetylneuraminic acid synthase (sialic acid synthase) |
| Gene symbol |
Nans |
| Other names/aliases |
4632418E04Rik
BB056474
Sas |
| Species |
Mus musculus |
| Database cross references - Nans |
| ExoCarta |
ExoCarta_94181 |
| Vesiclepedia |
VP_94181 |
| Entrez Gene |
94181 |
| Nans identified in sEVs derived from the following tissue/cell type |
|
Brain cancer cells
|
19109410
|
|
Colon cancer cells
|
37309723
|
|
T-cell lymphoma cells
|
37309723
|
| Gene ontology annotations for Nans |
|
|
| Experiment description of studies that identified Nans in sEVs |
| 1 |
| Experiment ID |
26 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Alix|GAPDH|HSP70|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
19109410
|
| Organism |
Mus musculus |
| Experiment description |
Proteomic and immunologic analyses of brain tumor exosomes. |
| Authors |
"Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD" |
| Journal name |
FASEB
|
| Publication year |
2008 |
| Sample |
Brain cancer cells |
| Sample name |
SMA560vIII |
| Isolation/purification methods |
Differential centrifugation
Sucrose density gradient |
| Flotation density |
1.13-1.15 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [MALDI TOF/TOF]
Western blotting |
|
|
| 2 |
| Experiment ID |
907 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
|
Enriched markers |
|
✔
HSPA5
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
37309723
|
| Organism |
Mus musculus |
| Experiment description |
Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells |
| Authors |
"Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
Colon cancer cells |
| Sample name |
C26 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 3 |
| Experiment ID |
908 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
|
Enriched markers |
|
✔
HSPA5
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
37309723
|
| Organism |
Mus musculus |
| Experiment description |
Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells |
| Authors |
"Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
T-cell lymphoma cells |
| Sample name |
EL4 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| Protein-protein interactions for Nans |
|
|
| Pathways in which Nans is involved |
|
|
|
