Gene description for CD86
Gene name CD86 molecule
Gene symbol CD86
Other names/aliases B7-2
B7.2
B70
CD28LG2
LAB72
Species Homo sapiens
 Database cross references - CD86
ExoCarta ExoCarta_942
Vesiclepedia VP_942
Entrez Gene 942
HGNC 1705
MIM 601020
UniProt P42081  
 CD86 identified in sEVs derived from the following tissue/cell type
B cell lymphoblastoid cells 10878338    
B cells 9685355    
B cells 17641064    
Breast milk 17641064    
Breast milk 17641064    
Bronchoalveolar lavage fluid 14582906    
Dendritic cells 14582906    
 Gene ontology annotations for CD86
Molecular Function
    virus receptor activity GO:0001618 IEA
    protein binding GO:0005515 IPI
    coreceptor activity GO:0015026 NAS
    signaling receptor activity GO:0038023 TAS
    receptor ligand activity GO:0048018 IDA
Biological Process
    adaptive immune response GO:0002250 IEA
    positive regulation of immunoglobulin production GO:0002639 ISS
    immune response GO:0006955 IBA
    cell surface receptor signaling pathway GO:0007166 IBA
    positive regulation of cell population proliferation GO:0008284 TAS
    T cell costimulation GO:0031295 IBA
    positive regulation of interleukin-2 production GO:0032743 NAS
    positive regulation of interleukin-4 production GO:0032753 NAS
    positive regulation of lymphotoxin A production GO:0032761 NAS
    positive regulation of T cell proliferation GO:0042102 IBA
    T cell activation GO:0042110 IDA
    B cell activation GO:0042113 ISS
    negative regulation of T cell proliferation GO:0042130 IBA
    positive regulation of T-helper 2 cell differentiation GO:0045630 NAS
    positive regulation of DNA-templated transcription GO:0045893 NAS
    symbiont entry into host cell GO:0046718 IEA
    cellular response to lipopolysaccharide GO:0071222 IBA
    positive regulation of non-canonical NF-kappaB signal transduction GO:1901224 ISS
Subcellular Localization
    plasma membrane GO:0005886 IC
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    external side of plasma membrane GO:0009897 IBA
    cell surface GO:0009986 HDA
    centriolar satellite GO:0034451 IDA
    extracellular exosome GO:0070062 HDA
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified CD86 in sEVs
1
Experiment ID 37
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 10878338    
Organism Homo sapiens
Experiment description Direct immunosuppressive effects of EBV-encoded latent membrane protein 1.
Authors "Dukers DF, Meij P, Vervoort MB, Vos W, Scheper RJ, Meijer CJ, Bloemena E, Middeldorp JM"
Journal name JIMMU
Publication year 2000
Sample B cell lymphoblastoid cells
Sample name JY
B95-8
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
2
Experiment ID 16
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 9685355    
Organism Homo sapiens
Experiment description Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes.
Authors "Escola JM, Kleijmeer MJ, Stoorvogel W, Griffith JM, Yoshie O, Geuze HJ"
Journal name JBC
Publication year 1998
Sample B cells
Sample name RN (HLA-DR15+)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.12-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Immunoelectron Microscopy
3
Experiment ID 47
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 17641064    
Organism Homo sapiens
Experiment description Exosomes with immune modulatory features are present in human breast milk.
Authors "Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S"
Journal name JIMMU
Publication year 2007
Sample B cells
Sample name B cells
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study FACS
4
Experiment ID 46
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 17641064    
Organism Homo sapiens
Experiment description Exosomes with immune modulatory features are present in human breast milk.
Authors "Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S"
Journal name JIMMU
Publication year 2007
Sample Breast milk
Sample name Breast milk - Colostrum
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.10-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
FACS
5
Experiment ID 48
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 17641064    
Organism Homo sapiens
Experiment description Exosomes with immune modulatory features are present in human breast milk.
Authors "Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S"
Journal name JIMMU
Publication year 2007
Sample Breast milk
Sample name Breast milk - Mature milk
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.10-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
FACS
6
Experiment ID 49
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 14582906    
Organism Homo sapiens
Experiment description Exosomes with major histocompatibility complex class II and co-stimulatory molecules are present in human BAL fluid.
Authors "Admyre C, Grunewald J, Thyberg J, Gripenback S, Tornling G, Eklund A, Scheynius A, Gabrielsson S"
Journal name ERJ
Publication year 2003
Sample Bronchoalveolar lavage fluid
Sample name Bronchoalveolar lavage fluid
Isolation/purification methods Differential centrifugation
Immunobeads (EpCAM)
Flotation density -
Molecules identified in the study Protein
Methods used in the study FACS
Immunoelectron Microscopy
7
Experiment ID 50
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 14582906    
Organism Homo sapiens
Experiment description Exosomes with major histocompatibility complex class II and co-stimulatory molecules are present in human BAL fluid.
Authors "Admyre C, Grunewald J, Thyberg J, Gripenback S, Tornling G, Eklund A, Scheynius A, Gabrielsson S"
Journal name ERJ
Publication year 2003
Sample Dendritic cells
Sample name Monocyte-derived dendritic cells
Isolation/purification methods Differential centrifugation
Immunobeads (EpCAM)
Flotation density -
Molecules identified in the study Protein
Methods used in the study FACS
Immunoelectron Microscopy
 Protein-protein interactions for CD86
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD80 941
Co-localization Homo sapiens
2 MARCH8  
Affinity Capture-Western Homo sapiens
3 PARP1 142
Two-hybrid Homo sapiens
4 CTLA4  
Co-crystal Structure Homo sapiens
Co-localization Homo sapiens
5 ECE2  
Two-hybrid Homo sapiens
6 MARCH1  
Affinity Capture-Western Homo sapiens
7 DDX58 23586
Affinity Capture-RNA Homo sapiens
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