Gene description for SERPINA3
Gene name serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3
Gene symbol SERPINA3
Other names/aliases AACT
ACT
GIG24
GIG25
Species Homo sapiens
 Database cross references - SERPINA3
ExoCarta ExoCarta_12
Vesiclepedia VP_12
Entrez Gene 12
HGNC 16
MIM 107280
UniProt P01011  
 SERPINA3 identified in sEVs derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells 36408942    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Thymus 23844026    
Urine 15326289    
Urine 19056867    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for SERPINA3
Molecular Function
    DNA binding GO:0003677 TAS
    serine-type endopeptidase inhibitor activity GO:0004867 TAS
    protein binding GO:0005515 IPI
Biological Process
    acute-phase response GO:0006953 IEA
    inflammatory response GO:0006954 NAS
    regulation of lipid metabolic process GO:0019216 NAS
    maintenance of gastrointestinal epithelium GO:0030277 NAS
Subcellular Localization
    extracellular region GO:0005576 NAS
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    nucleus GO:0005634 TAS
    platelet alpha granule lumen GO:0031093 TAS
    secretory granule lumen GO:0034774 TAS
    azurophil granule lumen GO:0035578 TAS
    collagen-containing extracellular matrix GO:0062023 HDA
    extracellular exosome GO:0070062 HDA
    blood microparticle GO:0072562 HDA
 Experiment description of studies that identified SERPINA3 in sEVs
1
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 258
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 259
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 13
MISEV standards
✔
IEM
Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101|CD9|AQP2|AQP1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors "Pisitkun T, Shen RF, Knepper MA"
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
11
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
12
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
14
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SERPINA3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NFAM1  
Affinity Capture-MS Homo sapiens
2 HBB 3043
Affinity Capture-MS Homo sapiens
3 STATH  
Affinity Capture-MS Homo sapiens
4 NRSN1  
Affinity Capture-MS Homo sapiens
5 SKI 6497
Affinity Capture-MS Homo sapiens
6 GC 2638
Affinity Capture-MS Homo sapiens
7 EBF2  
Affinity Capture-MS Homo sapiens
8 ZIC1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 MED24 9862
Affinity Capture-MS Homo sapiens
10 AGPAT1 10554
Affinity Capture-MS Homo sapiens
11 ZNF674 641339
Affinity Capture-MS Homo sapiens
12 KLK2  
Reconstituted Complex Homo sapiens
13 HPX 3263
Affinity Capture-MS Homo sapiens
14 HP 3240
Affinity Capture-MS Homo sapiens
15 ZDHHC6  
Affinity Capture-MS Homo sapiens
16 ORM1 5004
Affinity Capture-MS Homo sapiens
17 EGFL8  
Affinity Capture-MS Homo sapiens
18 NUDT3 11165
Affinity Capture-MS Homo sapiens
19 FMNL2 114793
Affinity Capture-MS Homo sapiens
20 SFXN4  
Affinity Capture-MS Homo sapiens
21 THAP10  
Affinity Capture-MS Homo sapiens
22 TCEB3B  
Affinity Capture-MS Homo sapiens
23 PHF11 51131
Affinity Capture-MS Homo sapiens
24 SERPINA1 5265
Affinity Capture-MS Homo sapiens
25 SNX27 81609
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 TF 7018
Affinity Capture-MS Homo sapiens
27 TWF1 5756
Affinity Capture-MS Homo sapiens
28 C18orf21  
Affinity Capture-MS Homo sapiens
29 ABCC5 10057
Two-hybrid Homo sapiens
30 DDX19B 11269
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 TRIM41 90933
Affinity Capture-MS Homo sapiens
32 DDX31  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 GNG8  
Affinity Capture-MS Homo sapiens
34 WHSC1 7468
Affinity Capture-MS Homo sapiens
35 CLU 1191
Affinity Capture-MS Homo sapiens
36 RNF115  
Affinity Capture-MS Homo sapiens
37 FAM227B  
Affinity Capture-MS Homo sapiens
38 SERPINC1 462
Cross-Linking-MS (XL-MS) Homo sapiens
39 SARAF  
Affinity Capture-MS Homo sapiens
40 MIA3 375056
Affinity Capture-MS Homo sapiens
41 SSUH2  
Affinity Capture-MS Homo sapiens
42 GDPD1 284161
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 RPA4  
Proximity Label-MS Homo sapiens
44 LCN1 3933
Affinity Capture-MS Homo sapiens
45 ATP6V1B1 525
Affinity Capture-MS Homo sapiens
46 ELANE 1991
Biochemical Activity Homo sapiens
47 HCN1  
Affinity Capture-MS Homo sapiens
48 APTX  
Affinity Capture-MS Homo sapiens
49 UPK1A 11045
Affinity Capture-MS Homo sapiens
50 GJB5  
Affinity Capture-MS Homo sapiens
51 FSTL1 11167
Affinity Capture-MS Homo sapiens
52 LAMC3  
Two-hybrid Homo sapiens
53 MRM1  
Affinity Capture-MS Homo sapiens
54 RHBDD1 84236
Affinity Capture-MS Homo sapiens
55 PSMA3 5684
Affinity Capture-MS Homo sapiens
56 PPP2R2B 5521
Affinity Capture-MS Homo sapiens
57 RABGGTA 5875
Affinity Capture-MS Homo sapiens
58 FANCD2  
Affinity Capture-MS Homo sapiens
59 AIFM1 9131
Affinity Capture-MS Homo sapiens
60 CCNC  
Affinity Capture-MS Homo sapiens
61 LMNB1 4001
Two-hybrid Homo sapiens
62 C16orf70  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 FAM60A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 TBC1D22B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 TMEM176B 28959
Affinity Capture-MS Homo sapiens
66 MOGAT3  
Affinity Capture-MS Homo sapiens
67 DDX60 55601
Affinity Capture-MS Homo sapiens
68 PCP4  
Affinity Capture-MS Homo sapiens
69 ATP6AP2 10159
Affinity Capture-MS Homo sapiens
70 CP 1356
Affinity Capture-MS Homo sapiens
71 PLD5  
Affinity Capture-MS Homo sapiens
72 CTSG 1511
Affinity Capture-MS Homo sapiens
73 PRR4 11272
Affinity Capture-MS Homo sapiens
74 RPS2P32  
Affinity Capture-MS Homo sapiens
75 NFKB1 4790
Two-hybrid Homo sapiens
76 MPI 4351
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 SLC37A4 2542
Affinity Capture-MS Homo sapiens
78 STX17 55014
Affinity Capture-MS Homo sapiens
79 UBE2U  
Affinity Capture-MS Homo sapiens
80 PRSS16 10279
Affinity Capture-MS Homo sapiens
81 CIR1  
Affinity Capture-MS Homo sapiens
82 PVRL2 5819
Affinity Capture-MS Homo sapiens
83 SCGB1D1  
Affinity Capture-MS Homo sapiens
84 SH2D3A  
Affinity Capture-MS Homo sapiens
85 GABPA 2551
Affinity Capture-MS Homo sapiens
86 EMC4 51234
Affinity Capture-MS Homo sapiens
87 FGB 2244
Affinity Capture-MS Homo sapiens
88 CDK15  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 PDE6D 5147
Affinity Capture-MS Homo sapiens
90 KLHL11  
Affinity Capture-MS Homo sapiens
91 MBNL1 4154
Affinity Capture-MS Homo sapiens
92 TEFM  
Affinity Capture-MS Homo sapiens
93 PINK1  
Affinity Capture-MS Homo sapiens
94 RAB11A 8766
Affinity Capture-MS Homo sapiens
95 GOT1 2805
Affinity Capture-MS Homo sapiens
96 ADH1B 125
Two-hybrid Homo sapiens
97 ZNRD1  
Two-hybrid Homo sapiens
98 DNAJC1 64215
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
99 OR2A4 79541
Affinity Capture-MS Homo sapiens
100 MATN2 4147
Affinity Capture-MS Homo sapiens
101 CCR1  
Affinity Capture-MS Homo sapiens
102 P2RX6  
Affinity Capture-MS Homo sapiens
103 APOA1 335
Cross-Linking-MS (XL-MS) Homo sapiens
View the network image/svg+xml



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