Gene description for Casp1
Gene name caspase 1
Gene symbol Casp1
Other names/aliases ICE
Il1bc
Species Mus musculus
 Database cross references - Casp1
ExoCarta ExoCarta_12362
Vesiclepedia VP_12362
Entrez Gene 12362
UniProt P29452  
 Casp1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Casp1
Molecular Function
    endopeptidase activity GO:0004175 ISO
    cysteine-type endopeptidase activity GO:0004197 IDA
    cysteine-type endopeptidase activity GO:0004197 ISO
    protein binding GO:0005515 IPI
    peptidase activity GO:0008233 IDA
    kinase binding GO:0019900 ISO
    cytokine binding GO:0019955 ISO
    identical protein binding GO:0042802 ISO
    CARD domain binding GO:0050700 ISO
    scaffold protein binding GO:0097110 ISO
Biological Process
    response to hypoxia GO:0001666 IMP
    response to hypoxia GO:0001666 ISO
    microglial cell activation GO:0001774 ISO
    positive regulation of cytokine production GO:0001819 IDA
    positive regulation of cytokine production GO:0001819 ISO
    pattern recognition receptor signaling pathway GO:0002221 NAS
    proteolysis GO:0006508 ISO
    apoptotic process GO:0006915 IEA
    osmosensory signaling pathway GO:0007231 NAS
    myoblast fusion GO:0007520 ISO
    memory GO:0007613 ISO
    response to xenobiotic stimulus GO:0009410 ISO
    response to bacterium GO:0009617 IMP
    regulation of autophagy GO:0010506 IMP
    macrophage cytokine production GO:0010934 IMP
    response to organic cyclic compound GO:0014070 ISO
    protein processing GO:0016485 IDA
    protein processing GO:0016485 IMP
    protein autoprocessing GO:0016540 IDA
    protein autoprocessing GO:0016540 ISO
    protein autoprocessing GO:0016540 ISS
    response to lipopolysaccharide GO:0032496 IDA
    response to lipopolysaccharide GO:0032496 ISO
    interleukin-1 alpha production GO:0032610 IMP
    interleukin-1 beta production GO:0032611 IGI
    interleukin-1 beta production GO:0032611 IMP
    positive regulation of interleukin-1 alpha production GO:0032730 IMP
    positive regulation of interleukin-1 beta production GO:0032731 IDA
    positive regulation of interleukin-1 beta production GO:0032731 IMP
    positive regulation of interleukin-1 beta production GO:0032731 ISO
    positive regulation of interleukin-1 beta production GO:0032731 NAS
    positive regulation of interleukin-18 production GO:0032741 ISO
    response to ATP GO:0033198 IMP
    defense response to bacterium GO:0042742 IDA
    regulation of apoptotic process GO:0042981 IEA
    positive regulation of apoptotic process GO:0043065 ISO
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IDA
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 ISO
    positive regulation of circadian sleep/wake cycle, non-REM sleep GO:0046010 ISO
    regulation of inflammatory response GO:0050727 IBA
    regulation of inflammatory response GO:0050727 IMP
    regulation of inflammatory response GO:0050727 ISO
    regulation of inflammatory response GO:0050727 ISS
    positive regulation of inflammatory response GO:0050729 IDA
    positive regulation of inflammatory response GO:0050729 ISO
    positive regulation of inflammatory response GO:0050729 NAS
    defense response to Gram-negative bacterium GO:0050829 IMP
    protein maturation GO:0051604 IDA
    protein maturation GO:0051604 ISO
    defense response to virus GO:0051607 NAS
    mitochondrial depolarization GO:0051882 IMP
    membrane hyperpolarization GO:0060081 IMP
    positive regulation of macrophage cytokine production GO:0060907 IMP
    pyroptotic inflammatory response GO:0070269 IDA
    pyroptotic inflammatory response GO:0070269 IMP
    pyroptotic inflammatory response GO:0070269 ISO
    pyroptotic inflammatory response GO:0070269 ISS
    pyroptotic inflammatory response GO:0070269 NAS
    cellular response to lipopolysaccharide GO:0071222 ISO
    cellular response to type II interferon GO:0071346 ISO
    programmed necrotic cell death GO:0097300 IDA
    cytokine precursor processing GO:0140447 ISO
    signaling receptor ligand precursor processing GO:0140448 ISO
    AIM2 inflammasome complex assembly GO:0140970 IDA
    AIM2 inflammasome complex assembly GO:0140970 ISO
    positive regulation of tumor necrosis factor-mediated signaling pathway GO:1903265 ISO
    positive regulation of cysteine-type endopeptidase activity GO:2001056 ISO
Subcellular Localization
    extracellular region GO:0005576 IDA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 NAS
    cytoplasm GO:0005737 TAS
    mitochondrion GO:0005739 IEA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 TAS
    microtubule GO:0005874 NAS
    plasma membrane GO:0005886 IEA
    protein-containing complex GO:0032991 ISO
    canonical inflammasome complex GO:0061702 ISO
    IPAF inflammasome complex GO:0072557 IBA
    IPAF inflammasome complex GO:0072557 IDA
    NLRP1 inflammasome complex GO:0072558 ISO
    NLRP1 inflammasome complex GO:0072558 ISS
    NLRP1 inflammasome complex GO:0072558 NAS
    NLRP3 inflammasome complex GO:0072559 IBA
    NLRP3 inflammasome complex GO:0072559 ISO
    NLRP3 inflammasome complex GO:0072559 ISS
    AIM2 inflammasome complex GO:0097169 IBA
    AIM2 inflammasome complex GO:0097169 ISO
    AIM2 inflammasome complex GO:0097169 ISS
    protease inhibitor complex GO:0097179 ISO
 Experiment description of studies that identified Casp1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Casp1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fancd2  
Affinity Capture-MS Mus musculus
2 Usp5 22225
Affinity Capture-Western Mus musculus
3 Tnfaip3  
Affinity Capture-Western Mus musculus
4 Il1b  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
5 Tmem173  
Proximity Label-MS Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here