Gene description for Usp5
Gene name ubiquitin specific peptidase 5 (isopeptidase T)
Gene symbol Usp5
Other names/aliases AA407472
ISOT
ISOT-1
Ucht
Species Mus musculus
 Database cross references - Usp5
ExoCarta ExoCarta_22225
Vesiclepedia VP_22225
Entrez Gene 22225
UniProt P56399  
 Usp5 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Neural stem cells 25242146    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Usp5
Molecular Function
    cysteine-type deubiquitinase activity GO:0004843 IBA
    cysteine-type deubiquitinase activity GO:0004843 IEA
    cysteine-type deubiquitinase activity GO:0004843 ISO
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IEA
    ubiquitin binding GO:0043130 IEA
    ubiquitin binding GO:0043130 ISO
    deubiquitinase activity GO:0101005 ISO
Biological Process
    proteolysis GO:0006508 IEA
    regulation of protein stability GO:0031647 IBA
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 IEA
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 ISO
    protein K48-linked deubiquitination GO:0071108 IEA
    protein K48-linked deubiquitination GO:0071108 ISO
    regulation protein catabolic process at presynapse GO:0140251 EXP
    regulation protein catabolic process at presynapse GO:0140251 IDA
    regulation protein catabolic process at presynapse GO:0140251 IEP
    regulation protein catabolic process at presynapse GO:0140251 IMP
    negative regulation of ubiquitin-dependent protein catabolic process GO:2000059 IEA
    negative regulation of ubiquitin-dependent protein catabolic process GO:2000059 ISO
Subcellular Localization
    nucleus GO:0005634 IBA
    cytosol GO:0005829 IBA
    presynapse GO:0098793 IEA
 Experiment description of studies that identified Usp5 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 263
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th1 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Usp5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Pycard  
Affinity Capture-Western Mus musculus
2 Kctd13  
Affinity Capture-MS Mus musculus
3 Ubc  
Reconstituted Complex Mus musculus
Reconstituted Complex Mus musculus
4 Herc4  
Co-fractionation Mus musculus
5 Pdcd1 18566
Affinity Capture-Western Mus musculus
6 Eed  
Affinity Capture-MS Mus musculus
7 Isg15  
Reconstituted Complex Mus musculus
8 Nlrp3  
Affinity Capture-Western Mus musculus
9 Usp7  
Co-fractionation Mus musculus
10 Mapt  
Affinity Capture-MS Mus musculus
11 MTNR1A  
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
12 Atg16l1  
Affinity Capture-MS Mus musculus
13 Gnpda1  
Co-fractionation Mus musculus
14 Fancd2  
Affinity Capture-MS Mus musculus
15 Casp1 12362
Affinity Capture-Western Mus musculus
16 PDCD1  
Biochemical Activity Homo sapiens
17 Twf1 19230
Co-fractionation Mus musculus
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