Gene description for Gspt1
Gene name G1 to S phase transition 1
Gene symbol Gspt1
Other names/aliases AI314175
AI326449
AV307676
C79774
G1st
Gst-1
Species Mus musculus
 Database cross references - Gspt1
ExoCarta ExoCarta_14852
Vesiclepedia VP_14852
Entrez Gene 14852
UniProt Q8R050  
 Gspt1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Gspt1
Molecular Function
    translation release factor activity GO:0003747 IBA
    translation release factor activity GO:0003747 ISO
    translation release factor activity GO:0003747 ISS
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IEA
    GTPase activity GO:0003924 ISO
    GTP binding GO:0005525 IEA
Biological Process
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 IEA
    translation GO:0006412 IBA
    translational termination GO:0006415 ISO
    regulation of translational termination GO:0006449 ISO
    regulation of translational termination GO:0006449 ISS
    protein methylation GO:0006479 ISO
Subcellular Localization
    cytoplasm GO:0005737 NAS
    translation release factor complex GO:0018444 IBA
    translation release factor complex GO:0018444 ISO
    cytosolic ribosome GO:0022626 IEA
    cytosolic ribosome GO:0022626 ISO
 Experiment description of studies that identified Gspt1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Gspt1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RBM45  
Affinity Capture-MS Homo sapiens
2 ZBTB11  
Affinity Capture-MS Homo sapiens
3 PABPC4 8761
Affinity Capture-MS Homo sapiens
4 ABCG1  
Affinity Capture-MS Homo sapiens
5 LARP4B 23185
Affinity Capture-MS Homo sapiens
6 SHROOM3 57619
Affinity Capture-MS Homo sapiens
7 CHST12 55501
Affinity Capture-MS Homo sapiens
8 EIF3I 8668
Affinity Capture-MS Homo sapiens
9 PPP1R13L 10848
Affinity Capture-MS Homo sapiens
10 EIF4G1 1981
Affinity Capture-MS Homo sapiens
11 CCDC86  
Affinity Capture-MS Homo sapiens
12 Atxn1  
Affinity Capture-MS Mus musculus
13 Foxp3  
Affinity Capture-MS Mus musculus
14 P4HA1 5033
Affinity Capture-MS Homo sapiens
15 NPAT  
Affinity Capture-MS Homo sapiens
16 MRPL27 51264
Affinity Capture-MS Homo sapiens
17 ADNP 23394
Affinity Capture-MS Homo sapiens
18 ETF1 2107
Affinity Capture-MS Homo sapiens
19 GTPBP1 9567
Affinity Capture-MS Homo sapiens
20 HSCB 150274
Affinity Capture-MS Homo sapiens
21 NTHL1  
Affinity Capture-MS Homo sapiens
22 METAP1D  
Affinity Capture-MS Homo sapiens
23 Tmem173  
Proximity Label-MS Mus musculus
24 PNO1 56902
Affinity Capture-MS Homo sapiens
25 ERCC3  
Affinity Capture-MS Homo sapiens
26 TTC31  
Affinity Capture-MS Homo sapiens
27 CSPG4 1464
Affinity Capture-MS Homo sapiens
28 PABPC1 26986
Affinity Capture-MS Homo sapiens
29 Cdkn2a  
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
30 RTCB 51493
Affinity Capture-MS Homo sapiens
31 TBL1XR1 79718
Affinity Capture-MS Homo sapiens
32 WDR74 54663
Affinity Capture-MS Homo sapiens
33 C14orf166 51637
Affinity Capture-MS Homo sapiens
34 ASCC2 84164
Affinity Capture-MS Homo sapiens
35 EEFSEC 60678
Affinity Capture-MS Homo sapiens
36 GOLM1 51280
Affinity Capture-MS Homo sapiens
37 PLXNA3 55558
Affinity Capture-MS Homo sapiens
38 HMGA1 3159
Affinity Capture-MS Homo sapiens
39 PFDN5 5204
Affinity Capture-MS Homo sapiens
40 BZW1 9689
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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