Gene description for Lat
Gene name linker for activation of T cells
Gene symbol Lat
Other names/aliases -
Species Mus musculus
 Database cross references - Lat
ExoCarta ExoCarta_16797
Vesiclepedia VP_16797
Entrez Gene 16797
UniProt O54957  
 Lat identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Lat
Molecular Function
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IBA
    protein kinase binding GO:0019901 IEA
    protein kinase binding GO:0019901 ISO
    signaling receptor complex adaptor activity GO:0030159 ISO
    signaling receptor complex adaptor activity GO:0030159 ISS
    molecular condensate scaffold activity GO:0140693 ISO
Biological Process
    adaptive immune response GO:0002250 IEA
    lymphocyte homeostasis GO:0002260 IMP
    inflammatory response GO:0006954 IBA
    inflammatory response GO:0006954 IMP
    immune response GO:0006955 IBA
    immune response GO:0006955 ISO
    immune response GO:0006955 ISS
    cellular defense response GO:0006968 TAS
    cell surface receptor protein tyrosine kinase signaling pathway GO:0007169 TAS
    integrin-mediated signaling pathway GO:0007229 ISO
    integrin-mediated signaling pathway GO:0007229 ISS
    Ras protein signal transduction GO:0007265 ISO
    Ras protein signal transduction GO:0007265 ISS
    gene expression GO:0010467 IMP
    calcium-mediated signaling GO:0019722 ISO
    calcium-mediated signaling GO:0019722 ISS
    membrane raft distribution GO:0031580 ISO
    intracellular signal transduction GO:0035556 IBA
    intracellular signal transduction GO:0035556 ISO
    intracellular signal transduction GO:0035556 ISS
    mast cell degranulation GO:0043303 IEA
    homeostasis of number of cells GO:0048872 IMP
    T cell receptor signaling pathway GO:0050852 IEA
    T cell receptor signaling pathway GO:0050852 ISO
    regulation of T cell activation GO:0050863 IBA
    regulation of T cell activation GO:0050863 ISO
    regulation of T cell activation GO:0050863 ISS
Subcellular Localization
    immunological synapse GO:0001772 IBA
    immunological synapse GO:0001772 IDA
    immunological synapse GO:0001772 ISO
    Golgi apparatus GO:0005794 IEA
    Golgi apparatus GO:0005794 ISO
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 TAS
    cell-cell junction GO:0005911 IDA
    COP9 signalosome GO:0008180 IDA
    membrane GO:0016020 ISO
    TCR signalosome GO:0036398 ISO
 Experiment description of studies that identified Lat in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Lat
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Foxp3  
Affinity Capture-MS Mus musculus
2 Usp9x 22284
Affinity Capture-Western Mus musculus
View the network image/svg+xml



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