Gene description for Usp9x
Gene name ubiquitin specific peptidase 9, X chromosome
Gene symbol Usp9x
Other names/aliases 5730589N07Rik
AA407302
AA407699
AL022658
AL022749
Dffrx
FAF-X
Fafl
Species Mus musculus
 Database cross references - Usp9x
ExoCarta ExoCarta_22284
Vesiclepedia VP_22284
Entrez Gene 22284
UniProt P70398  
 Usp9x identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
Pancreatic cells 19351151    
 Gene ontology annotations for Usp9x
Molecular Function
    cysteine-type endopeptidase activity GO:0004197 IDA
    cysteine-type deubiquitinase activity GO:0004843 IBA
    cysteine-type deubiquitinase activity GO:0004843 IDA
    cysteine-type deubiquitinase activity GO:0004843 ISO
    cysteine-type deubiquitinase activity GO:0004843 ISS
    protein binding GO:0005515 IPI
    ubiquitin protein ligase binding GO:0031625 IPI
    metal ion binding GO:0046872 IEA
    K63-linked deubiquitinase activity GO:0061578 ISO
    K63-linked deubiquitinase activity GO:0061578 ISS
    co-SMAD binding GO:0070410 ISO
    co-SMAD binding GO:0070410 ISS
    deubiquitinase activity GO:0101005 IDA
    deubiquitinase activity GO:0101005 ISO
    molecular sequestering activity GO:0140313 ISO
    K11-linked deubiquitinase activity GO:0180017 ISO
    K11-linked deubiquitinase activity GO:0180017 ISS
    K48-linked deubiquitinase activity GO:1990380 ISO
    K48-linked deubiquitinase activity GO:1990380 ISS
Biological Process
    in utero embryonic development GO:0001701 IGI
    neuron migration GO:0001764 IMP
    neuron migration GO:0001764 ISO
    DNA alkylation repair GO:0006307 ISO
    DNA alkylation repair GO:0006307 ISS
    chromosome segregation GO:0007059 IEA
    transforming growth factor beta receptor signaling pathway GO:0007179 ISO
    transforming growth factor beta receptor signaling pathway GO:0007179 ISS
    post-embryonic development GO:0009791 IMP
    cell migration GO:0016477 IBA
    protein import into peroxisome matrix, receptor recycling GO:0016562 ISO
    protein import into peroxisome matrix, receptor recycling GO:0016562 ISS
    protein deubiquitination GO:0016579 IMP
    protein deubiquitination GO:0016579 ISO
    protein deubiquitination GO:0016579 ISS
    cerebellar cortex structural organization GO:0021698 IMP
    hippocampus development GO:0021766 IMP
    BMP signaling pathway GO:0030509 ISO
    BMP signaling pathway GO:0030509 ISS
    regulation of protein stability GO:0031647 IBA
    positive regulation of protein binding GO:0032092 ISO
    positive regulation of protein binding GO:0032092 ISS
    negative regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032435 ISO
    negative regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032435 ISS
    monoubiquitinated protein deubiquitination GO:0035520 ISO
    monoubiquitinated protein deubiquitination GO:0035520 ISS
    regulation of circadian rhythm GO:0042752 ISO
    regulation of circadian rhythm GO:0042752 ISS
    rhythmic process GO:0048511 IEA
    axon extension GO:0048675 IMP
    axon extension GO:0048675 IMP
    axon extension GO:0048675 ISO
    protein stabilization GO:0050821 ISO
    protein stabilization GO:0050821 ISS
    regulation of T cell receptor signaling pathway GO:0050856 ISO
    cell division GO:0051301 IEA
    cilium assembly GO:0060271 ISO
    cytosolic ciliogenesis GO:0061824 ISO
    protein K63-linked deubiquitination GO:0070536 ISO
    protein K63-linked deubiquitination GO:0070536 ISS
    cellular response to transforming growth factor beta stimulus GO:0071560 IMP
    protein deubiquitination involved in ubiquitin-dependent protein catabolic process GO:0071947 IMP
    protein deubiquitination involved in ubiquitin-dependent protein catabolic process GO:0071947 ISO
    regulation of postsynapse assembly GO:0150052 IDA
    regulation of postsynapse assembly GO:0150052 IEP
    regulation of postsynapse assembly GO:0150052 IMP
    positive regulation of TORC2 signaling GO:1904515 ISO
    positive regulation of TORC2 signaling GO:1904515 ISS
    neuron projection extension GO:1990138 IGI
Subcellular Localization
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    centrosome GO:0005813 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    cytosol GO:0005829 ISS
    cytoskeleton GO:0005856 IEA
    cilium GO:0005929 ISO
    cilium GO:0005929 ISS
    growth cone GO:0030426 IEA
    growth cone GO:0030426 ISO
    apical part of cell GO:0045177 IDA
    postsynapse GO:0098794 IDA
 Experiment description of studies that identified Usp9x in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
2
Experiment ID 188
MISEV standards
EM
Biophysical techniques
GAPDH|UCHL1|HSP90
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Usp9x
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Lat 16797
Affinity Capture-Western Mus musculus
2 Fxr1 14359
Affinity Capture-MS Mus musculus
3 Ubc  
Affinity Capture-MS Mus musculus
Reconstituted Complex Mus musculus
4 Msr1  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
5 Prkcb  
Affinity Capture-Western Mus musculus
6 Fitm2  
Positive Genetic Mus musculus
7 Ctnnb1 12387
Affinity Capture-Western Mus musculus
8 Tcp1 21454
Co-fractionation Mus musculus
9 Tnik  
Affinity Capture-MS Mus musculus
10 Tsc1  
Affinity Capture-MS Mus musculus
11 Atxn1  
Proximity Label-MS Mus musculus
12 Kctd13  
Affinity Capture-MS Mus musculus
13 Htt  
Affinity Capture-Western Mus musculus
14 Chaf1a  
Affinity Capture-MS Mus musculus
15 Huwe1  
Co-fractionation Mus musculus
16 Dlgap1  
Affinity Capture-MS Mus musculus
17 Dcx  
Affinity Capture-Western Mus musculus
18 Agap2  
Affinity Capture-MS Mus musculus
19 Unk  
Affinity Capture-RNA Mus musculus
20 Snca  
Co-fractionation Mus musculus
21 Eed  
Affinity Capture-MS Mus musculus
22 Fmr1  
Affinity Capture-MS Mus musculus
23 Rptor  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
24 Sall4  
Affinity Capture-MS Mus musculus
25 Atg12  
Positive Genetic Mus musculus
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