Gene description for DHX36
Gene name DEAH (Asp-Glu-Ala-His) box polypeptide 36
Gene symbol DHX36
Other names/aliases DDX36
G4R1
MLEL1
RHAU
Species Homo sapiens
 Database cross references - DHX36
ExoCarta ExoCarta_170506
Vesiclepedia VP_170506
Entrez Gene 170506
HGNC 14410
MIM 612767
UniProt Q9H2U1  
 DHX36 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 34887515    
Embryonic kidney cells 36706192    
Embryonic kidney cells 36706192    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for DHX36
Molecular Function
    magnesium ion binding GO:0000287 IDA
    transcription cis-regulatory region binding GO:0000976 ISS
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IDA
    G-quadruplex RNA binding GO:0002151 IBA
    G-quadruplex RNA binding GO:0002151 IDA
    G-quadruplex RNA binding GO:0002151 IMP
    DNA helicase activity GO:0003678 IBA
    DNA helicase activity GO:0003678 IDA
    DNA clamp loader activity GO:0003689 IEA
    single-stranded DNA binding GO:0003697 IDA
    RNA binding GO:0003723 HDA
    RNA binding GO:0003723 IDA
    RNA helicase activity GO:0003724 IBA
    RNA helicase activity GO:0003724 IDA
    double-stranded RNA binding GO:0003725 IDA
    mRNA 3'-UTR binding GO:0003730 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 ISS
    ATP-dependent activity, acting on DNA GO:0008094 IDA
    four-way junction helicase activity GO:0009378 IEA
    ATP hydrolysis activity GO:0016887 IEA
    mRNA 3'-UTR AU-rich region binding GO:0035925 IDA
    double-stranded DNA helicase activity GO:0036121 IEA
    histone deacetylase binding GO:0042826 ISS
    mRNA 5'-UTR binding GO:0048027 IDA
    G-quadruplex DNA binding GO:0051880 IBA
    G-quadruplex DNA binding GO:0051880 IDA
    forked DNA-dependent helicase activity GO:0061749 IEA
    cohesin loader activity GO:0061775 IEA
    telomerase RNA binding GO:0070034 IDA
    pre-miRNA binding GO:0070883 ISS
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
    single-stranded 3'-5' DNA helicase activity GO:1990518 IEA
Biological Process
    ossification GO:0001503 ISS
    positive regulation of myeloid dendritic cell cytokine production GO:0002735 IEA
    chromatin remodeling GO:0006338 IEA
    regulation of transcription by RNA polymerase III GO:0006359 IMP
    spermatogenesis GO:0007283 ISS
    RNA secondary structure unwinding GO:0010501 IDA
    positive regulation of gene expression GO:0010628 ISS
    negative regulation of translation GO:0017148 IDA
    cell differentiation GO:0030154 IEA
    positive regulation of mRNA 3'-end processing GO:0031442 IMP
    positive regulation of telomere maintenance GO:0032206 IMP
    DNA duplex unwinding GO:0032508 IEA
    positive regulation of interferon-alpha production GO:0032727 IMP
    cellular response to heat GO:0034605 IDA
    cellular response to UV GO:0034644 IMP
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 ISS
    response to exogenous dsRNA GO:0043330 IEA
    regulation of mRNA stability GO:0043488 IMP
    G-quadruplex DNA unwinding GO:0044806 IDA
    innate immune response GO:0045087 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 ISS
    regulation of embryonic development GO:0045995 ISS
    defense response to virus GO:0051607 IEA
    positive regulation of cardioblast differentiation GO:0051891 ISS
    positive regulation of transcription initiation by RNA polymerase II GO:0060261 IMP
    positive regulation of dendritic spine morphogenesis GO:0061003 ISS
    3'-UTR-mediated mRNA destabilization GO:0061158 IMP
    telomerase RNA stabilization GO:0090669 IDA
    chromatin looping GO:0140588 IEA
    positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay GO:1900153 IMP
    positive regulation of hematopoietic progenitor cell differentiation GO:1901534 ISS
    cellular response to arsenite ion GO:1903843 IDA
    positive regulation of telomere maintenance via telomere lengthening GO:1904358 IMP
    positive regulation of intracellular mRNA localization GO:1904582 ISS
    positive regulation of cytoplasmic translation GO:2000767 IMP
Subcellular Localization
    chromosome, telomeric region GO:0000781 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    cytoplasmic stress granule GO:0010494 IDA
    nuclear speck GO:0016607 IDA
    axon GO:0030424 ISS
    dendrite GO:0030425 ISS
    perikaryon GO:0043204 ISS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified DHX36 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
11
Experiment ID 1275
MISEV standards
Biophysical techniques
✔
CD9|TSG101|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 1280
MISEV standards
Biophysical techniques
✔
TSG101|CD9|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T - Fraction 5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density 1.151 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
13
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
14
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
15
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
16
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
17
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
18
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DHX36
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GATA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 ZBTB38 253461
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 NUPL2 11097
Affinity Capture-MS Homo sapiens
4 ZNF668  
Affinity Capture-MS Homo sapiens
5 H2AFJ 55766
Affinity Capture-MS Homo sapiens
6 RPSA 3921
Affinity Capture-MS Homo sapiens
7 HNRNPC 3183
Affinity Capture-MS Homo sapiens
8 RPL26L1 51121
Affinity Capture-MS Homo sapiens
9 HEBP1 50865
Co-fractionation Homo sapiens
10 SLFN11 91607
Affinity Capture-MS Homo sapiens
11 ZNF346  
Affinity Capture-MS Homo sapiens
12 RIT1 6016
Negative Genetic Homo sapiens
13 TARDBP 23435
Affinity Capture-MS Homo sapiens
14 RPA2 6118
Affinity Capture-MS Homo sapiens
15 RPSAP58 388524
Affinity Capture-MS Homo sapiens
16 RPS19 6223
Affinity Capture-MS Homo sapiens
17 BYSL 705
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 SSB 6741
Affinity Capture-MS Homo sapiens
19 CAND1 55832
Affinity Capture-MS Homo sapiens
20 FGF3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 RPS3 6188
Affinity Capture-MS Homo sapiens
22 ILF2 3608
Affinity Capture-MS Homo sapiens
23 HNRNPUL2 221092
Affinity Capture-MS Homo sapiens
24 APEX1 328
Affinity Capture-RNA Homo sapiens
25 GSPT2 23708
Affinity Capture-MS Homo sapiens
26 HEATR3 55027
Affinity Capture-MS Homo sapiens
27 RBM3 5935
Affinity Capture-MS Homo sapiens
28 KIF23 9493
Affinity Capture-MS Homo sapiens
29 COPS5 10987
Affinity Capture-MS Homo sapiens
30 Cep85l  
Affinity Capture-MS Mus musculus
31 GDI1 2664
Co-fractionation Homo sapiens
32 NPM1 4869
Affinity Capture-MS Homo sapiens
33 PARVA 55742
Affinity Capture-MS Homo sapiens
34 Mib1  
Two-hybrid Mus musculus
35 RBM4B  
Affinity Capture-MS Homo sapiens
36 ABT1 29777
Affinity Capture-MS Homo sapiens
37 SEPT7 989
Co-fractionation Homo sapiens
38 MECP2 4204
Affinity Capture-MS Homo sapiens
39 Vps28 66914
Affinity Capture-MS Mus musculus
40 MYCN  
Affinity Capture-MS Homo sapiens
41 FAM120A 23196
Affinity Capture-MS Homo sapiens
42 MTERF3  
Proximity Label-MS Homo sapiens
43 GSK3B 2932
Affinity Capture-MS Homo sapiens
44 RBM19 9904
Affinity Capture-MS Homo sapiens
45 LARP4B 23185
Affinity Capture-MS Homo sapiens
46 ACP6 51205
Affinity Capture-MS Homo sapiens
47 LIN28A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 RBMX 27316
Affinity Capture-MS Homo sapiens
49 UNK  
Affinity Capture-RNA Homo sapiens
50 E2F4  
Affinity Capture-MS Homo sapiens
51 AURKA 6790
Affinity Capture-MS Homo sapiens
52 LIN28B  
Affinity Capture-MS Homo sapiens
53 RPL9 6133
Cross-Linking-MS (XL-MS) Homo sapiens
54 PARK2  
Affinity Capture-MS Homo sapiens
55 RPS10 6204
Affinity Capture-MS Homo sapiens
56 SRSF3 6428
Affinity Capture-MS Homo sapiens
57 YBX2 51087
Affinity Capture-MS Homo sapiens
58 ZGRF1 55345
Cross-Linking-MS (XL-MS) Homo sapiens
59 MAPRE1 22919
Affinity Capture-MS Homo sapiens
60 SRSF6 6431
Affinity Capture-MS Homo sapiens
61 PURG  
Affinity Capture-MS Homo sapiens
62 S100A6 6277
Co-fractionation Homo sapiens
63 HIST1H2AM 8336
Affinity Capture-MS Homo sapiens
64 RPL5 6125
Affinity Capture-MS Homo sapiens
65 AKR7L 246181
Affinity Capture-MS Homo sapiens
66 FUBP1 8880
Co-fractionation Homo sapiens
67 DDX5 1655
Co-fractionation Homo sapiens
68 NTRK1 4914
Affinity Capture-MS Homo sapiens
69 FGF8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 HIST1H1E 3008
Affinity Capture-MS Homo sapiens
71 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
72 TBRG4 9238
Proximity Label-MS Homo sapiens
73 HIST1H1C 3006
Affinity Capture-MS Homo sapiens
74 RPL27A 6157
Affinity Capture-MS Homo sapiens
75 TAGLN2 8407
Co-fractionation Homo sapiens
76 DHX9 1660
Affinity Capture-MS Homo sapiens
77 HECTD1 25831
Affinity Capture-MS Homo sapiens
78 MAGEB2 4113
Affinity Capture-MS Homo sapiens
79 PRKAB1 5564
Affinity Capture-MS Homo sapiens
80 PAIP1 10605
Affinity Capture-MS Homo sapiens
81 ANLN 54443
Affinity Capture-MS Homo sapiens
82 FGFBP1 9982
Affinity Capture-MS Homo sapiens
83 RECQL5  
Co-fractionation Homo sapiens
84 RPL31 6160
Affinity Capture-MS Homo sapiens
85 UBE2H 7328
Affinity Capture-MS Homo sapiens
86 PANK2 80025
Affinity Capture-MS Homo sapiens
87 TRIM31  
Affinity Capture-MS Homo sapiens
88 RPS2 6187
Affinity Capture-MS Homo sapiens
89 RPL15 6138
Affinity Capture-MS Homo sapiens
90 RC3H2  
Affinity Capture-MS Homo sapiens
91 ECT2 1894
Affinity Capture-MS Homo sapiens
92 SIRT7  
Affinity Capture-MS Homo sapiens
93 SRSF1 6426
Affinity Capture-MS Homo sapiens
94 ATG12  
Affinity Capture-MS Homo sapiens
95 CUL3 8452
Affinity Capture-MS Homo sapiens
96 RICTOR 253260
Affinity Capture-MS Homo sapiens
97 SRSF5 6430
Affinity Capture-MS Homo sapiens
98 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 MYC  
Affinity Capture-MS Homo sapiens
100 Max  
Affinity Capture-MS Mus musculus
101 ZNF777  
Affinity Capture-MS Homo sapiens
102 PRR3  
Affinity Capture-MS Homo sapiens
103 PCBP1 5093
Affinity Capture-MS Homo sapiens
104 IL7R  
Protein-RNA Homo sapiens
105 PSPC1 55269
Affinity Capture-MS Homo sapiens
106 RPA3 6119
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
107 RPS13 6207
Affinity Capture-MS Homo sapiens
108 PABPC5 140886
Affinity Capture-MS Homo sapiens
109 ILK 3611
Affinity Capture-MS Homo sapiens
110 Rpl35 66489
Affinity Capture-MS Mus musculus
111 H2AFB2  
Affinity Capture-MS Homo sapiens
112 MRPS17 51373
Affinity Capture-MS Homo sapiens
113 FSCN1 6624
Affinity Capture-MS Homo sapiens
114 HINT1 3094
Co-fractionation Homo sapiens
115 HNRNPH1 3187
Affinity Capture-MS Homo sapiens
116 RBMS2 5939
Affinity Capture-MS Homo sapiens
117 DDX55  
Affinity Capture-MS Homo sapiens
118 RPS16 6217
Affinity Capture-MS Homo sapiens
119 RPL19 6143
Affinity Capture-MS Homo sapiens
120 ZNF574  
Affinity Capture-MS Homo sapiens
121 DZIP3  
Affinity Capture-MS Homo sapiens
122 RPL13 6137
Affinity Capture-MS Homo sapiens
123 DHX15 1665
Co-fractionation Homo sapiens
124 SURF6  
Affinity Capture-MS Homo sapiens
125 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
126 FASN 2194
Negative Genetic Homo sapiens
127 PDGFB  
Affinity Capture-MS Homo sapiens
128 ZFP36  
Two-hybrid Homo sapiens
129 TP53 7157
Affinity Capture-MS Homo sapiens
130 WWOX 51741
Affinity Capture-MS Homo sapiens
131 APOBEC3D  
Affinity Capture-MS Homo sapiens
132 RPA1 6117
Affinity Capture-MS Homo sapiens
133 MEN1 4221
Affinity Capture-MS Homo sapiens
134 ASB12  
Affinity Capture-MS Homo sapiens
135 G3BP1 10146
Affinity Capture-Western Homo sapiens
136 C1orf35  
Affinity Capture-MS Homo sapiens
137 CPEB1  
Affinity Capture-MS Homo sapiens
138 DDX58 23586
Affinity Capture-Western Homo sapiens
139 MKI67  
Affinity Capture-MS Homo sapiens
140 CCDC93 54520
Affinity Capture-MS Homo sapiens
141 ESR1  
Affinity Capture-MS Homo sapiens
142 UFL1 23376
Affinity Capture-MS Homo sapiens
143 KRR1 11103
Affinity Capture-MS Homo sapiens
144 NEIL1  
Affinity Capture-MS Homo sapiens
145 RPS24 6229
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
146 Mbd2  
Affinity Capture-MS Mus musculus
147 EZH2  
Affinity Capture-MS Homo sapiens
148 SRSF7 6432
Affinity Capture-MS Homo sapiens
149 RPS6 6194
Affinity Capture-MS Homo sapiens
150 PTGES3 10728
Co-fractionation Homo sapiens
151 RPL23A 6147
Affinity Capture-MS Homo sapiens
152 KRAS 3845
Negative Genetic Homo sapiens
153 HNRNPU 3192
Affinity Capture-MS Homo sapiens
154 C9orf72  
Affinity Capture-MS Homo sapiens
155 RC3H1 149041
Affinity Capture-MS Homo sapiens
156 IVNS1ABP  
Affinity Capture-MS Homo sapiens
157 HARS 3035
Co-fractionation Homo sapiens
158 HECW2  
Affinity Capture-MS Homo sapiens
159 HIST1H2AE 3012
Affinity Capture-MS Homo sapiens
160 RPL17 6139
Affinity Capture-MS Homo sapiens
161 EP300 2033
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here