Gene description for Nme1
Gene name NME/NM23 nucleoside diphosphate kinase 1
Gene symbol Nme1
Other names/aliases AL024257
NDPK-A
NM23-M1
NM23A
Species Mus musculus
 Database cross references - Nme1
ExoCarta ExoCarta_18102
Vesiclepedia VP_18102
Entrez Gene 18102
UniProt P15532  
 Nme1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Neural stem cells 25242146    
Oligodendrocytes 21136642    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Nme1
Molecular Function
    magnesium ion binding GO:0000287 IEA
    magnesium ion binding GO:0000287 ISO
    RNA polymerase II transcription regulatory region sequence-specific DNA binding GO:0000977 ISO
    single-stranded DNA binding GO:0003697 ISO
    DNA nuclease activity GO:0004536 IEA
    DNA nuclease activity GO:0004536 ISO
    nucleoside diphosphate kinase activity GO:0004550 IBA
    nucleoside diphosphate kinase activity GO:0004550 ISO
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    GTP binding GO:0005525 IEA
    GTP binding GO:0005525 ISO
    3'-5' exonuclease activity GO:0008408 IEA
    3'-5' exonuclease activity GO:0008408 ISO
    intermediate filament binding GO:0019215 ISO
    enzyme binding GO:0019899 ISO
    protein kinase binding GO:0019901 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    gamma-tubulin binding GO:0043015 ISO
    ribosomal small subunit binding GO:0043024 IEA
    ribosomal small subunit binding GO:0043024 ISO
Biological Process
    negative regulation of myeloid leukocyte differentiation GO:0002762 ISO
    GTP biosynthetic process GO:0006183 IEA
    GTP biosynthetic process GO:0006183 ISO
    UTP biosynthetic process GO:0006228 IEA
    dTMP biosynthetic process GO:0006231 NAS
    CTP biosynthetic process GO:0006241 IEA
    DNA metabolic process GO:0006259 IEA
    endocytosis GO:0006897 IEA
    nervous system development GO:0007399 IEA
    lactation GO:0007595 IMP
    negative regulation of gene expression GO:0010629 ISO
    positive regulation of neuron projection development GO:0010976 ISO
    cell differentiation GO:0030154 IEA
    mammary gland development GO:0030879 IMP
    positive regulation of DNA binding GO:0043388 ISO
    positive regulation of epithelial cell proliferation GO:0050679 IEA
    positive regulation of epithelial cell proliferation GO:0050679 ISO
Subcellular Localization
    nucleus GO:0005634 IEA
    nucleus GO:0005634 ISO
    mitochondrion GO:0005739 HDA
    mitochondrial outer membrane GO:0005741 ISO
    early endosome GO:0005769 IEA
    early endosome GO:0005769 ISO
    centrosome GO:0005813 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    intermediate filament GO:0005882 ISO
    ruffle membrane GO:0032587 IEA
    ruffle membrane GO:0032587 ISO
    myelin sheath GO:0043209 HDA
    perinuclear region of cytoplasm GO:0048471 ISO
 Experiment description of studies that identified Nme1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 263
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th1 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 33
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Nme1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fancd2  
Affinity Capture-MS Mus musculus
2 Strap 20901
Co-fractionation Mus musculus
3 Thop1  
Co-fractionation Mus musculus
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