Gene description for Pfkm
Gene name phosphofructokinase, muscle
Gene symbol Pfkm
Other names/aliases AI131669
ATP-PFK
PFK-A
PFK-M
Pfk-4
Pfk4
Pfka
Pfkx
Species Mus musculus
 Database cross references - Pfkm
ExoCarta ExoCarta_18642
Vesiclepedia VP_18642
Entrez Gene 18642
UniProt P47857  
 Pfkm identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Pfkm
Molecular Function
    6-phosphofructokinase activity GO:0003872 IBA
    6-phosphofructokinase activity GO:0003872 IDA
    6-phosphofructokinase activity GO:0003872 IMP
    6-phosphofructokinase activity GO:0003872 ISO
    6-phosphofructokinase activity GO:0003872 ISS
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    phosphofructokinase activity GO:0008443 IDA
    phosphofructokinase activity GO:0008443 ISO
    AMP binding GO:0016208 ISO
    kinase binding GO:0019900 IEA
    kinase binding GO:0019900 ISO
    carbohydrate binding GO:0030246 ISO
    identical protein binding GO:0042802 IPI
    identical protein binding GO:0042802 ISO
    metal ion binding GO:0046872 IEA
    fructose binding GO:0070061 IEA
    fructose binding GO:0070061 ISO
    fructose-6-phosphate binding GO:0070095 IBA
    fructose-6-phosphate binding GO:0070095 ISO
Biological Process
    glycogen catabolic process GO:0005980 IMP
    fructose 6-phosphate metabolic process GO:0006002 IBA
    fructose 6-phosphate metabolic process GO:0006002 IEA
    fructose 6-phosphate metabolic process GO:0006002 ISO
    glycolytic process GO:0006096 ISO
    fructose 1,6-bisphosphate metabolic process GO:0030388 IBA
    fructose 1,6-bisphosphate metabolic process GO:0030388 ISO
    positive regulation of insulin secretion GO:0032024 IMP
    glucose homeostasis GO:0042593 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    muscle cell cellular homeostasis GO:0046716 IEA
    muscle cell cellular homeostasis GO:0046716 ISO
    carbohydrate phosphorylation GO:0046835 IEA
    glycolytic process through fructose-6-phosphate GO:0061615 IC
    glycolytic process through fructose-6-phosphate GO:0061615 IC
    glycolytic process through fructose-6-phosphate GO:0061615 IDA
    glycolytic process through fructose-6-phosphate GO:0061615 IDA
    glycolytic process through fructose-6-phosphate GO:0061615 IMP
    glycolytic process through fructose-6-phosphate GO:0061615 ISO
    canonical glycolysis GO:0061621 IBA
    canonical glycolysis GO:0061621 IDA
    canonical glycolysis GO:0061621 IGI
    canonical glycolysis GO:0061621 IMP
    glycolysis from storage polysaccharide through glucose-1-phosphate GO:0093001 IMP
Subcellular Localization
    nucleus GO:0005634 IEA
    nucleus GO:0005634 ISO
    cytosol GO:0005829 IDA
    cytosol GO:0005829 IDA
    6-phosphofructokinase complex GO:0005945 IBA
    6-phosphofructokinase complex GO:0005945 IEA
    6-phosphofructokinase complex GO:0005945 ISO
    membrane GO:0016020 IBA
    apical plasma membrane GO:0016324 IEA
    apical plasma membrane GO:0016324 ISO
    sperm principal piece GO:0097228 IDA
 Experiment description of studies that identified Pfkm in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Pfkm
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tubb2a  
Co-fractionation Mus musculus
2 Tpm2  
Co-fractionation Mus musculus
3 Pfkm 18642
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
4 Kcna3  
Affinity Capture-MS Mus musculus
5 Kctd13  
Affinity Capture-MS Mus musculus
6 MAPT  
Reconstituted Complex Homo sapiens
7 Mapt  
Affinity Capture-MS Mus musculus
8 Tia1  
Affinity Capture-MS Mus musculus
9 Slc25a4 11739
Co-fractionation Mus musculus
10 Ywhaz 22631
Affinity Capture-MS Mus musculus
11 Cycs 13063
Co-fractionation Mus musculus
12 Usp13  
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Pfkm is involved
PathwayEvidenceSource
Glucose metabolism IEA Reactome
Glycolysis IEA Reactome
Metabolism IEA Reactome
Metabolism of carbohydrates IEA Reactome





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