Gene description for Slit2
Gene name slit homolog 2 (Drosophila)
Gene symbol Slit2
Other names/aliases Drad-1
E030015M03Rik
E130320P19Rik
Slil3
mKIAA4141
slit-2
Species Mus musculus
 Database cross references - Slit2
ExoCarta ExoCarta_20563
Vesiclepedia VP_20563
Entrez Gene 20563
UniProt Q9R1B9  
 Slit2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Slit2
Molecular Function
    GTPase inhibitor activity GO:0005095 ISO
    signaling receptor binding GO:0005102 TAS
    extracellular matrix structural constituent GO:0005201 RCA
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    heparin binding GO:0008201 IBA
    heparin binding GO:0008201 IEA
    heparin binding GO:0008201 ISO
    identical protein binding GO:0042802 ISO
    protein homodimerization activity GO:0042803 ISO
    laminin-1 binding GO:0043237 ISO
    proteoglycan binding GO:0043394 ISO
    heparan sulfate proteoglycan binding GO:0043395 ISO
    chemorepellent activity GO:0045499 IMP
    Roundabout binding GO:0048495 IBA
    Roundabout binding GO:0048495 ISO
Biological Process
    metanephros development GO:0001656 IMP
    ureteric bud development GO:0001657 ISO
    in utero embryonic development GO:0001701 IMP
    kidney development GO:0001822 IMP
    negative regulation of protein phosphorylation GO:0001933 ISO
    cell migration involved in sprouting angiogenesis GO:0002042 ISO
    negative regulation of leukocyte chemotaxis GO:0002689 ISO
    aortic valve morphogenesis GO:0003180 IMP
    pulmonary valve morphogenesis GO:0003184 IMP
    axonogenesis GO:0007409 IDA
    axonogenesis GO:0007409 IMP
    axon guidance GO:0007411 IDA
    axon guidance GO:0007411 IGI
    axon guidance GO:0007411 IMP
    axon guidance GO:0007411 ISO
    motor neuron axon guidance GO:0008045 ISO
    negative regulation of cell population proliferation GO:0008285 IGI
    negative regulation of lamellipodium assembly GO:0010593 ISO
    negative regulation of endothelial cell migration GO:0010596 ISO
    negative regulation of gene expression GO:0010629 IGI
    negative regulation of smooth muscle cell migration GO:0014912 ISO
    olfactory bulb development GO:0021772 IGI
    chemorepulsion involved in postnatal olfactory bulb interneuron migration GO:0021836 ISO
    corticospinal neuron axon guidance through spinal cord GO:0021972 ISO
    telencephalon cell migration GO:0022029 IGI
    negative regulation of cell growth GO:0030308 IBA
    negative regulation of cell growth GO:0030308 ISO
    negative regulation of cell migration GO:0030336 ISO
    negative regulation of axon extension GO:0030517 IDA
    negative regulation of actin filament polymerization GO:0030837 ISO
    retinal ganglion cell axon guidance GO:0031290 IBA
    retinal ganglion cell axon guidance GO:0031290 IGI
    retinal ganglion cell axon guidance GO:0031290 IMP
    retinal ganglion cell axon guidance GO:0031290 ISO
    dorsal/ventral axon guidance GO:0033563 IGI
    dorsal/ventral axon guidance GO:0033563 IMP
    Roundabout signaling pathway GO:0035385 ISO
    positive regulation of apoptotic process GO:0043065 ISO
    negative regulation of vascular permeability GO:0043116 ISO
    branching morphogenesis of an epithelial tube GO:0048754 ISO
    neuron projection morphogenesis GO:0048812 IGI
    neuron projection morphogenesis GO:0048812 IMP
    axon extension involved in axon guidance GO:0048846 ISO
    negative chemotaxis GO:0050919 IBA
    negative chemotaxis GO:0050919 IMP
    negative chemotaxis GO:0050919 ISO
    induction of negative chemotaxis GO:0050929 ISO
    negative regulation of small GTPase mediated signal transduction GO:0051058 ISO
    positive regulation of synapse assembly GO:0051965 ISO
    ventricular septum morphogenesis GO:0060412 IMP
    mammary gland duct morphogenesis GO:0060603 IGI
    mammary gland duct morphogenesis GO:0060603 IMP
    mammary duct terminal end bud growth GO:0060763 IMP
    negative regulation of chemokine-mediated signaling pathway GO:0070100 ISO
    cellular response to heparin GO:0071504 ISO
    negative regulation of smooth muscle cell chemotaxis GO:0071672 ISO
    negative regulation of mononuclear cell migration GO:0071676 ISO
    negative regulation of neutrophil chemotaxis GO:0090024 ISO
    negative regulation of monocyte chemotaxis GO:0090027 ISO
    negative regulation of retinal ganglion cell axon guidance GO:0090260 ISO
    negative regulation of cellular response to growth factor stimulus GO:0090288 ISO
    cell-cell adhesion GO:0098609 IGI
    cell-cell adhesion GO:0098609 IMP
    neuron projection extension GO:1990138 ISO
Subcellular Localization
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IDA
    extracellular space GO:0005615 ISO
    cytoplasm GO:0005737 ISO
    plasma membrane GO:0005886 TAS
    cell surface GO:0009986 ISO
    axon GO:0030424 ISO
    dendrite GO:0030425 ISO
    cell projection GO:0042995 ISO
    neuronal cell body GO:0043025 ISO
    cell body GO:0044297 ISO
    collagen-containing extracellular matrix GO:0062023 HDA
 Experiment description of studies that identified Slit2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Slit2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Robo4  
Co-fractionation Mus musculus
2 Robo3  
Affinity Capture-Western Mus musculus
3 Ctnnb1 12387
Co-localization Mus musculus
4 Robo1  
Co-fractionation Mus musculus
View the network image/svg+xml



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