Gene description for ALOX12
Gene name arachidonate 12-lipoxygenase
Gene symbol ALOX12
Other names/aliases 12-LOX
12S-LOX
LOG12
Species Homo sapiens
 Database cross references - ALOX12
ExoCarta ExoCarta_239
Vesiclepedia VP_239
Entrez Gene 239
HGNC 429
MIM 152391
UniProt P18054  
 ALOX12 identified in sEVs derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Urine 19056867    
 Gene ontology annotations for ALOX12
Molecular Function
    arachidonate 12(S)-lipoxygenase activity GO:0004052 IBA
    arachidonate 12(S)-lipoxygenase activity GO:0004052 IDA
    iron ion binding GO:0005506 IEA
    protein binding GO:0005515 IPI
    linoleate 13S-lipoxygenase activity GO:0016165 IBA
    linoleate 13S-lipoxygenase activity GO:0016165 IDA
    hepoxilin-epoxide hydrolase activity GO:0047977 ISS
    arachidonate 15-lipoxygenase activity GO:0050473 IBA
    arachidonate 15-lipoxygenase activity GO:0050473 IDA
Biological Process
    lipid metabolic process GO:0006629 IDA
    negative regulation of muscle cell apoptotic process GO:0010656 IMP
    arachidonate metabolic process GO:0019369 IBA
    arachidonate metabolic process GO:0019369 IDA
    arachidonate metabolic process GO:0019369 ISS
    lipoxygenase pathway GO:0019372 IBA
    lipoxygenase pathway GO:0019372 IDA
    fatty acid oxidation GO:0019395 IDA
    fatty acid oxidation GO:0019395 IMP
    unsaturated fatty acid metabolic process GO:0033559 IDA
    lipid oxidation GO:0034440 IBA
    superoxide anion generation GO:0042554 NAS
    linoleic acid metabolic process GO:0043651 IDA
    linoleic acid metabolic process GO:0043651 IDA
    hepoxilin biosynthetic process GO:0051122 ISS
    establishment of skin barrier GO:0061436 ISS
    negative regulation of platelet aggregation GO:0090331 ISS
    leukotriene A4 metabolic process GO:1901751 IDA
    leukotriene A4 metabolic process GO:1901751 IDA
    lipoxin A4 biosynthetic process GO:2001303 IDA
    lipoxin B4 biosynthetic process GO:2001306 IDA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    membrane GO:0016020 IDA
    sarcolemma GO:0042383 IBA
    sarcolemma GO:0042383 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified ALOX12 in sEVs
1
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for ALOX12
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 ACTA2 59
Affinity Capture-Western Homo sapiens
3 TRIB1 10221
Two-hybrid Homo sapiens
4 PLA2G2A 5320
Affinity Capture-Western Homo sapiens
5 MYCN  
Affinity Capture-MS Homo sapiens
6 FASN 2194
Negative Genetic Homo sapiens
7 KRT5 3852
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
8 ALOX12 239
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
9 LMNA 4000
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
10 PLA2G4A 5321
Affinity Capture-Western Homo sapiens
11 ITGB4 3691
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml



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